- 专利标题: Regulation of gene expression using chromatin remodelling factors
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申请号: US10516753申请日: 2003-06-06
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公开(公告)号: US07582807B2公开(公告)日: 2009-09-01
- 发明人: Abdelali Hannoufa , Derek J. Lydiate , Ming-Jun Gao
- 申请人: Abdelali Hannoufa , Derek J. Lydiate , Ming-Jun Gao
- 申请人地址: CA Saskatoon
- 专利权人: Her Majesty the Queen in right of Canada, as represented by the Minister of Agriculture and Agri-Food
- 当前专利权人: Her Majesty the Queen in right of Canada, as represented by the Minister of Agriculture and Agri-Food
- 当前专利权人地址: CA Saskatoon
- 代理机构: Merchant & Gould P.C.
- 国际申请: PCT/CA03/00822 WO 20030606
- 国际公布: WO03/104462 WO 20031218
- 主分类号: C12N15/82
- IPC分类号: C12N15/82 ; C12N15/63 ; C12N15/10 ; A01H5/00
摘要:
The invention provides a method to regulate expression of a gene of interest in a plant comprising, introducing into the plant a first nucleotide sequence comprising, the gene of interest operatively linked to a first regulatory region, and an operator sequence capable of binding a fusion protein, and a second nucleotide sequence comprising a second regulatory region in operative association with a nucleotide sequence encoding the fusion protein. The fusion protein comprising, a DNA binding protein, or a portion thereof, capable of binding the operator sequence, and a recruitment factor protein, or a portion thereof, capable of binding a chromatin remodelling protein. In this manner, expression of the second nucleotide sequence produces the fusion protein that regulates expression of the gene of interest.
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