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公开(公告)号：US07582807B2
公开(公告)日：2009-09-01
申请号：US10516753
申请日：2003-06-06
CPC分类号： C07K14/415 , C12N15/63 , C12N15/8216 , C12N15/8217
摘要： The invention provides a method to regulate expression of a gene of interest in a plant comprising, introducing into the plant a first nucleotide sequence comprising, the gene of interest operatively linked to a first regulatory region, and an operator sequence capable of binding a fusion protein, and a second nucleotide sequence comprising a second regulatory region in operative association with a nucleotide sequence encoding the fusion protein. The fusion protein comprising, a DNA binding protein, or a portion thereof, capable of binding the operator sequence, and a recruitment factor protein, or a portion thereof, capable of binding a chromatin remodelling protein. In this manner, expression of the second nucleotide sequence produces the fusion protein that regulates expression of the gene of interest.
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公开(公告)号：US20100088781A1
公开(公告)日：2010-04-08
申请号：US12528079
申请日：2008-02-21
CPC分类号： C12N15/825 , C12N9/0071
摘要： A method for altering the level of one or more carotenoids in a plant or a tissue within the plant is provided. Method comprises introducing a nucleic acid sequence comprising a regulatory region operatively associated with a silencing nucleotide sequence that reduces or eliminates the expression of a lycopene epsilon cyclase into the plant, and expressing the silencing nucleotide sequence. Expression of the sequence reduces the level of the lycopene epsilon cyclase in the plant or within a tissue of the plant, and results in altering one or more carotenoids.
摘要翻译： 提供了改变植物或植物内的组织中一种或多种类胡萝卜素水平的方法。 方法包括引入包含与沉默核苷酸序列可操作地相关联的调节区的核酸序列，其减少或消除番茄红素ε环化酶在植物中的表达，并表达沉默核苷酸序列。 该序列的表达降低植物中或植物组织内番茄红素ε环化酶的水平，并导致改变一种或多种类胡萝卜素。
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3.发明授权Repressor-mediated regulation system for control of gene expression in plants 失效抑制因子调控系统，用于控制植物中的基因表达公开(公告)号：US07521595B2
公开(公告)日：2009-04-21
申请号：US10995951
申请日：2004-11-23
申请人： Abdelali Hannoufa , Dwayne Hegedus , Nicholas Bate
发明人： Abdelali Hannoufa , Dwayne Hegedus , Nicholas Bate
CPC分类号： C12N15/8217 , C07K14/195
摘要： The invention provides a method for selectively controlling the transcription of a gene of interest, comprising producing one or more plants that express either a first, a second, or both the first and second genetic constructs. The first genetic construct comprises a first regulatory region operatively linked to a gene of interest and at least one repressor sequence capable of controlling the activity of the first regulatory region. The second genetic construct comprises a second regulatory region in operative association with a nucleic acid molecule, or a derivative thereof, encoding a repressor protein, the repressor protein exhibiting both repressor operator sequence binding activity and repressor activity.
摘要翻译： 本发明提供了选择性控制感兴趣的基因的转录的方法，其包括产生表达第一和第二遗传构建体的第一个，第二个或第二个的一个或多个植物。 第一遗传构建物包含与感兴趣的基因有效连接的第一调节区和至少一个能够控制第一调节区的活性的阻遏物序列。 第二遗传构建体包含与编码阻抑蛋白的核酸分子或其衍生物有效结合的第二调控区，所述阻遏蛋白具有阻遏物操纵子序列结合活性和阻遏物活性。
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公开(公告)号：US07321077B2
公开(公告)日：2008-01-22
申请号：US10719996
申请日：2003-11-21
申请人： Abdelali Hannoufa , Dwayne Hegedus , Nicholas Bate
发明人： Abdelali Hannoufa , Dwayne Hegedus , Nicholas Bate
CPC分类号： C12N15/8217 , C07K14/195
摘要： The invention provides a method for selectively controlling the transcription of a gene of interest, comprising producing one or more plants that express either a first, a second, or both the first and second genetic constructs. The first genetic construct comprises a first regulator region operatively linked to a gene of interest and at least one repressor sequence capable of controlling the activity of the first regulatory region. The second genetic construct comprises a second regulator region in operative association with a nucleic acid molecule, or a derivative thereof, encoding a repressor protein, the repressor protein exhibiting both repressor operator sequence binding activity and repressor activity. The first and second genetic constructs may reside on separate vectors, or the vector may comprise both the first and second genetic constructs comprised as just defined. If the first and second constructs reside within separate plants, then the first plant and the second plant are crossed to obtain progeny, so that the progeny comprise both the first genetic construct and the second genetic construct. The progeny of this cross are characterized in that the expression of the second genetic construct represses expression of the gene of interest. The first and second regulatory regions may be either the same or different and may be selected from the group consisting of a constitutive promoter, an inducible promoter, a tissue specific promoter, and a developmental promoter. if the plant comprises the vector that comprises both the first and second genetic construct, or if a plant has been co-transformed with the first and second genetic construct so that both the first and second genetic constructs may be expressed in the same plant, then it is preferred that the first and second regulatory regions are different. The first regulatory region may comprise a constitutive promoter, an inducible promoter, a tissue specific promoter, or a developmental promoter. The second regulatory region may comprise an inducible promoter, a tissue specific promoter, or a developmental promoter.
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公开(公告)号：US20070174933A1
公开(公告)日：2007-07-26
申请号：US11338849
申请日：2006-01-25
申请人： Abdelali Hannoufa , Ulrike Schafer
发明人： Abdelali Hannoufa , Ulrike Schafer
CPC分类号： C12Y203/01091 , C12N9/0073 , C12N9/1007 , C12N9/1029 , C12N9/88 , C12N15/8243 , C12Y114/13011 , C12Y201/01068 , C12Y403/01024
摘要： A method for reducing the level of one or more than one protein in a plant or a tissue within the plant, and plants with reduced levels of target proteins are provided. The method involves introducing a nucleic acid sequence into the plant, where the nucleic acid sequence comprises a regulatory region operatively associated with a silencing nucleotide sequence. The expression of the silencing nucleotide sequence reduces or eliminates the expression of two or more than two enzymes involved in the synthesis of the one or more than one protein. The reduced level of the one or more than one protein may be determine by comparing the level of the one or more than one protein in the plant, or a tissue of the plant, with a level of the one ore more than one protein in a second plant, or the tissue from the second plant, that does not express the nucleic acid sequence. Plants and seeds obtained from plants with reduced levels of target proteins, and reduced anti-nutrient factors are also provided
摘要翻译： 提供了降低植物或植物内的组织中的一种或多于一种蛋白质的水平的方法，以及降低靶蛋白水平的植物。 该方法包括将核酸序列引入植物中，其中核酸序列包含与沉默核苷酸序列可操作地相关联的调节区。 沉默核苷酸序列的表达降低或消除了参与合成一种或多于一种蛋白质的两种或两种以上的酶的表达。 一种或多于一种蛋白质的降低的水平可以通过将植物或植物组织中的一种或多种蛋白质的水平与一种或多种蛋白质的水平在一 第二植物或来自第二植物的组织，其不表达核酸序列。 还提供了从具有降低的靶蛋白水平的植物获得的植物和种子以及降低的抗营养因子
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公开(公告)号：US20050245732A1
公开(公告)日：2005-11-03
申请号：US10995951
申请日：2004-11-23
申请人： Abdelali Hannoufa , Dwayne Hegedus , Nicholas Bate
发明人： Abdelali Hannoufa , Dwayne Hegedus , Nicholas Bate
CPC分类号： C12N15/8217 , C07K14/195
摘要： The invention provides a method for selectively controlling the transcription of a gene of interest, comprising producing one or more plants that express either a first, a second, or both the first and second genetic constructs. The first genetic construct comprises a first regulatory region operatively linked to a gene of interest and at least one repressor sequence capable of controlling the activity of the first regulatory region. The second genetic construct comprises a second regulatory region in operative association with a nucleic acid molecule, or a derivative thereof, encoding a repressor protein, the repressor protein exhibiting both repressor operator sequence binding activity and repressor activity. The first and second genetic constructs may reside on separate vectors, or the vector may comprise both the first and second genetic constructs comprised as just defined. If the first and second constructs reside within separate plants, then the first plant and the second plant are crossed to obtain progeny, so that the progeny comprise both the first genetic construct and the second genetic construct. The progeny of this cross are characterized in that the expression of the second genetic construct represses expression of the gene of interest. The first and second regulatory regions may be either the same or different and may be selected from the group consisting of a constitutive promoter, an inducible promoter, a tissue specific promoter, and a developmental promoter. If the plant comprises the vector that comprises both the first and second genetic construct, or if a plant has been co-transformed with the first and second genetic construct so that both the first and second genetic constructs may be expressed in the same plant, then it is preferred that the first and second regulatory regions are different. The first regulatory region may comprise a constitutive promoter, an inducible promoter, a tissue specific promoter, or a developmental promoter. The second regulatory region may comprise an inducible promoter, a tissue specific promoter, or a developmental promoter.
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公开(公告)号：US07553667B2
公开(公告)日：2009-06-30
申请号：US11067425
申请日：2005-02-22
CPC分类号： C12N15/8217
摘要： The invention provides a method to regulate expression of a gene of interest in a plant comprising, introducing into the plant a first nucleotide sequence comprising, the gene of interest operatively linked to a first regulatory region, and an operator sequence capable of binding a fusion protein, and a second nucleotide sequence comprising a second regulatory region in operative association with a nucleotide sequence encoding the fusion protein. The fusion protein comprising, a DNA binding protein, or a portion thereof, capable of binding the operator sequence, and a recruitment factor protein, or a portion thereof, capable of binding a chromatin remodelling protein. In this manner, expression of the second nucleotide sequence produces the fusion protein that regulates expression of the gene of interest.
摘要翻译： 本发明提供了调节植物中目的基因表达的方法，包括：向植物中引入第一核苷酸序列，其包含可操作地连接到第一调节区的目的基因和能够结合融合蛋白的操纵子序列 和包含与编码融合蛋白的核苷酸序列有效结合的第二调节区的第二核苷酸序列。 包含能够结合操作者序列的DNA结合蛋白或其部分的融合蛋白，以及能够结合染色质重塑蛋白的募集因子蛋白或其部分。 以这种方式，第二核苷酸序列的表达产生调节感兴趣的基因表达的融合蛋白。
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公开(公告)号：US20060123506A1
公开(公告)日：2006-06-08
申请号：US10516753
申请日：2003-06-06
申请人： Abdelali Hannoufa , Derek Lydiate , Ming-Jun Gao
发明人： Abdelali Hannoufa , Derek Lydiate , Ming-Jun Gao
CPC分类号： C07K14/415 , C12N15/63 , C12N15/8216 , C12N15/8217
摘要： The invention provides a method to regulate expression of a gene of interest in a plant comprising, introducing into the plant a first nucleotide sequence comprising, the gene of interest operatively linked to a first regulatory region, and an operator sequence capable of binding a fusion protein, and a second nucleotide sequence comprising a second regulatory region in operative association with a nucleotide sequence encoding the fusion protein. The fusion protein comprising, a DNA binding protein, or a portion thereof, capable of binding the operator sequence, and a recruitment factor protein, or a portion thereof, capable of binding a chromatin remodelling protein. In this manner, expression of the second nucleotide sequence produces the fusion protein that regulates expression of the gene of interest.
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公开(公告)号：US20050278809A1
公开(公告)日：2005-12-15
申请号：US11067425
申请日：2005-02-22
申请人： Abdelali Hannoufa , Derek Lydiate , Ming-Jun Gao
发明人： Abdelali Hannoufa , Derek Lydiate , Ming-Jun Gao
CPC分类号： C12N15/8217
摘要： The invention provides a method to regulate expression of a gene of interest in a plant comprising, introducing into the plant a first nucleotide sequence comprising, the gene of interest operatively linked to a first regulatory region, and an operator sequence capable of binding a fusion protein, and a second nucleotide sequence comprising a second regulatory region in operative association with a nucleotide sequence encoding the fusion protein. The fusion protein comprising, a DNA binding protein, or a portion thereof, capable of binding the operator sequence, and a recruitment factor protein, or a portion thereof, capable of binding a chromatin remodelling protein. In this manner, expression of the second nucleotide sequence produces the fusion protein that regulates expression of the gene of interest.
摘要翻译： 本发明提供了调节植物中目的基因表达的方法，包括：向植物中引入第一核苷酸序列，其包含可操作地连接到第一调节区的目的基因和能够结合融合蛋白的操纵子序列 和包含与编码融合蛋白的核苷酸序列有效结合的第二调节区的第二核苷酸序列。 包含能够结合操作者序列的DNA结合蛋白或其部分的融合蛋白，以及能够结合染色质重塑蛋白的募集因子蛋白或其部分。 以这种方式，第二核苷酸序列的表达产生调节感兴趣的基因表达的融合蛋白。
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