发明授权
- 专利标题: Method for trapping nucleic acids using divalent metal ions
- 专利标题(中): 用二价金属离子捕获核酸的方法
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申请号: US11660549申请日: 2005-08-18
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公开(公告)号: US07615347B2公开(公告)日: 2009-11-10
- 发明人: Tadashi Fukasawa , Hiroshi Kurokawa
- 申请人: Tadashi Fukasawa , Hiroshi Kurokawa
- 申请人地址: JP Osaka
- 专利权人: Asahi Kasei Kabushiki Kaisha
- 当前专利权人: Asahi Kasei Kabushiki Kaisha
- 当前专利权人地址: JP Osaka
- 代理机构: Birch, Stewart, Kolasch & Birch, LLP
- 优先权: JP2004-240726 20040820; JP2004-240727 20040820
- 国际申请: PCT/JP2005/015051 WO 20050818
- 国际公布: WO2006/019127 WO 20060223
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; G01N15/06 ; C07H21/00 ; G01N33/53
摘要:
A method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and having a pH of 12 or higher with the surface of the solid phase substrate on which divalent metal ions are immobilized, and a method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and a magnesium compound, of which pH is adjusted to be 12 or higher, with the surface of the solid phase substrate.
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