发明授权
- 专利标题: Method of preparing assay sample for discriminating bacteria by flow cytometer
- 专利标题(中): 用流式细胞仪分析细菌的方法
-
申请号: US10803667申请日: 2004-03-18
-
公开(公告)号: US07645594B2公开(公告)日: 2010-01-12
- 发明人: Yasuhiro Sakai , Yasuyuki Kawashima , Junya Inoue , Yoshiro Ikeuchi
- 申请人: Yasuhiro Sakai , Yasuyuki Kawashima , Junya Inoue , Yoshiro Ikeuchi
- 申请人地址: JP Hyogo
- 专利权人: Sysmex Corporation
- 当前专利权人: Sysmex Corporation
- 当前专利权人地址: JP Hyogo
- 代理机构: Cohen Pontani Lieberman & Pavane LLP
- 优先权: JP2000-334641 20001101
- 主分类号: C12Q1/04
- IPC分类号: C12Q1/04 ; G01N1/30
摘要:
A method of staining bacteria comprises: working a polymethine dye on a sample in the presence of a substance capable of reducing nitrite ions to stain bacteria in the sample. A method of detecting bacteria comprises the following steps of: (1) working a polymethine dye on a sample by a method as described above to stain bacteria in the sample, (2) introducing the thus treated sample into a detecting part of a flow cytometer and irradiating cells of the stained bacteria one by one with light to measure scattered light and fluorescent light emitted from each of the cells; and (3) discriminating the bacteria from other components in accordance with an intensity of a scattered light signal and an intensity of a fluorescent light signal or a pulse width reflecting the length of particles to count the bacteria.