发明授权
US07674621B2 Plasmids and phages for homologous recombination and methods of use
有权
用于同源重组的质粒和噬菌体及其使用方法
- 专利标题： Plasmids and phages for homologous recombination and methods of use
- 专利标题（中）： 用于同源重组的质粒和噬菌体及其使用方法
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申请号： US11134795申请日： 2005-05-20
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公开(公告)号： US07674621B2公开(公告)日： 2010-03-09
- 发明人: Donald L. Court , Simanti Datta , Nina Costantino
- 申请人： Donald L. Court , Simanti Datta , Nina Costantino
- 申请人地址： US DC Washington
- 专利权人： The United States of America as represented by the Department of Health and Human Services
- 当前专利权人： The United States of America as represented by the Department of Health and Human Services
- 当前专利权人地址： US DC Washington
- 代理机构： Klarquist Sparkman, LLP
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N15/63 ; C12N15/70
摘要：
Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria.
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