A display substrate includes a plurality of gate lines, a plurality of data lines, a gate signal-inputting unit, a first test unit, and a first dummy switching unit. The gate lines extend in a first direction. The data lines extend in a second direction intersected with the first direction. The gate signal-inputting unit is formed at a first end of each of the gate lines to apply gate signals to the gate lines. The first test unit is formed at a second end of each of the gate lines opposite to the first end applying a first test signal to the gate lines. The first dummy switching unit is formed between the gate signal-inputting unit and the first test unit and transferring the first test signal to the gate lines.