发明授权
- 专利标题: Trichoderma reesei G/11 xylanases with improved stability
- 专利标题(中): 具有改善稳定性的里氏木霉G / 11木聚糖酶
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申请号: US10912272申请日: 2004-08-05
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公开(公告)号: US07718411B1公开(公告)日: 2010-05-18
- 发明人: Fred Fenel , Ossi Turunen , Matti Leisola
- 申请人: Fred Fenel , Ossi Turunen , Matti Leisola
- 申请人地址: US CA Palo Alto
- 专利权人: Danisco US Inc.
- 当前专利权人: Danisco US Inc.
- 当前专利权人地址: US CA Palo Alto
- 主分类号: C12N9/24
- IPC分类号: C12N9/24 ; C12N9/42 ; C07H21/04
摘要:
The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
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