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公开(公告)号：US07718411B1
公开(公告)日：2010-05-18
申请号：US10912272
申请日：2004-08-05
申请人： Fred Fenel , Ossi Turunen , Matti Leisola
发明人： Fred Fenel , Ossi Turunen , Matti Leisola
CPC分类号： C12N9/2482 , C12Y302/01008
摘要： The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
摘要翻译： 本发明涉及蛋白质工程，尤其涉及家族G / 11木聚糖酶，以及编码所述酶的基因。 具体而言，本发明涉及里氏木霉XYNII基因，其编码内切-1,4-纤维素 - 木聚糖酶（EC 3.2.1.8）。 本发明描述了如何使用定点诱变来改进酶的性质以匹配其使用的工业条件。 蛋白质工程可用于提高木聚糖酶的热活性和热稳定性，并扩大其pH范围。
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公开(公告)号：US06271007B1
公开(公告)日：2001-08-07
申请号：US08194624
申请日：1994-02-07
申请人： Juha Apajalahti , Matti Leisola
发明人： Juha Apajalahti , Matti Leisola
IPC分类号： C12P758
摘要： The invention relates to novel yeast strains having a reduced ability to metabolize xylitol. The invention further relates to the use of said strains for the production of xylitol.
摘要翻译： 本发明涉及具有降低木糖醇代谢能力的新型酵母菌株。 本发明还涉及所述菌株用于生产木糖醇的用途。
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3.发明授权Method to improve the stability and broaden the pH range of family G/11 xylanases 有权提高G / 11木聚糖酶稳定性和扩大pH范围的方法公开(公告)号：US08426181B2
公开(公告)日：2013-04-23
申请号：US11963285
申请日：2007-12-21
申请人： Fred Fenel , Ossi Turunen , Matti Leisola
发明人： Fred Fenel , Ossi Turunen , Matti Leisola
CPC分类号： C12N9/2402 , C12N9/2482 , C12Y302/01008
摘要： The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
摘要翻译： 本发明涉及蛋白质工程，尤其涉及家族G / 11木聚糖酶，以及编码所述酶的基因。 具体而言，本发明涉及编码内切-1,4-β-木聚糖酶（EC 3.2.1.8）的里氏木霉XYNII基因。 本发明描述了如何使用定点诱变来改进酶的性质以匹配其使用的工业条件。 蛋白质工程可用于提高木聚糖酶的热活性和热稳定性，并扩大其pH范围。
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公开(公告)号：US20050176110A1
公开(公告)日：2005-08-11
申请号：US10516071
申请日：2003-05-30
申请人： Matti Leisola , Jouni Jokela
发明人： Matti Leisola , Jouni Jokela
IPC分类号： B01D20060101 , B01D15/08 , B01D15/18 , C07H1/06 , C07H1/08 , C07H21/04 , C12N9/90 , C12P19/30 , C12P19/38
CPC分类号： G01N30/482 , B01D15/424 , B01J20/262 , B01J20/267 , B01J20/285 , B01J2220/54
摘要： The invention relates to a process for chromatographic separation and purification of natural and synthetic nucleosides, deoxynucleosides and corresponding bases by using cross-linked xylose isomerase. In particular, nucleosides are separated from biological material, especially industrial side streams.
摘要翻译： 本发明涉及通过使用交联木糖异构酶对天然和合成核苷，脱氧核苷和相应碱基进行色谱分离和纯化的方法。 特别地，核苷与生物材料，特别是工业侧流分离。
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5.发明申请Method to Improve the Stability and Broaden the pH range of family G/11 xylanases 有权提高稳定性和扩大家族G / 11木聚糖酶pH范围的方法公开(公告)号：US20080171374A1
公开(公告)日：2008-07-17
申请号：US11963285
申请日：2007-12-21
申请人： Fred Fenel , Ossi Turunen , Matti Leisola
发明人： Fred Fenel , Ossi Turunen , Matti Leisola
CPC分类号： C12N9/2402 , C12N9/2482 , C12Y302/01008
摘要： The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
摘要翻译： 本发明涉及蛋白质工程，尤其涉及家族G / 11木聚糖酶，以及编码所述酶的基因。 具体而言，本发明涉及编码内切-1,4-β-木聚糖酶（EC 3.2.1.8）的里氏木霉XYNII基因。 本发明描述了如何使用定点诱变来改进酶的性质以匹配其使用的工业条件。 蛋白质工程可用于提高木聚糖酶的热活性和热稳定性，并扩大其pH范围。
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