发明授权
US07723093B2 Uracil-DNA glycosylase of Psychrobacter sp. HJ147 and use thereof
有权
Psychrobacter sp。的尿嘧啶-DNA糖基化酶 HJ147及其用途
- 专利标题： Uracil-DNA glycosylase of Psychrobacter sp. HJ147 and use thereof
- 专利标题（中）： Psychrobacter sp。的尿嘧啶-DNA糖基化酶 HJ147及其用途
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申请号： US11823123申请日： 2007-06-26
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公开(公告)号： US07723093B2公开(公告)日： 2010-05-25
- 发明人: Suk-Tae Kwon , Mi-Sun Lee , Gun-A Kim , Jung-Hyun Lee
- 申请人： Suk-Tae Kwon , Mi-Sun Lee , Gun-A Kim , Jung-Hyun Lee
- 申请人地址： KR Suwon, Gyeonggi-Do
- 专利权人： Sungkyunkwan University Foundation for Corporate Collaboration
- 当前专利权人： Sungkyunkwan University Foundation for Corporate Collaboration
- 当前专利权人地址： KR Suwon, Gyeonggi-Do
- 代理机构： Edwards Angell Palmer & Dodge LLP
- 代理商 Kongsik Kim
- 优先权： KR10-2007-0023976 20070312
- 主分类号： C12N9/24
- IPC分类号： C12N9/24 ; C12N9/00 ; C12N1/21 ; C12Q1/68 ; C12P21/06 ; C12N15/70 ; C12P19/34 ; C07H21/04
摘要：
The present invention provides uracil-DNA glycosylase (UDG) gene originating from Psychrobacter sp. HJ147, and amino acid sequences deduced from the gene; expression and purification of Psp HJ147 UDG gene in Escherichia coli; and characterization of UDG obtained therefrom, and the use thereof in a polymerase chain reaction (PCR). The UDG according to the present invention has a specific activity of excising uracil bases in a uracil-containing DNA substrates at a low temperature, and is easily heat-inactivated. It thus can effectively eliminate cross contamination and carry-over contamination of PCR templates often occurring after a PCR process using dUTP. Therefore, it is useful for increasing preciseness (elimination of false positives), purity and amplification efficiency of PCR.
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