公开(公告)号：US07723093B2

公开(公告)日：2010-05-25

申请号：US11823123

申请日：2007-06-26

Applicant: Suk-Tae Kwon ,  Mi-Sun Lee ,  Gun-A Kim ,  Jung-Hyun Lee

Inventor： Suk-Tae Kwon ,  Mi-Sun Lee ,  Gun-A Kim ,  Jung-Hyun Lee

IPC: C12N9/24 ,  C12N9/00 ,  C12N1/21 ,  C12Q1/68 ,  C12P21/06 ,  C12N15/70 ,  C12P19/34 ,  C07H21/04

CPC classification number: C12Y302/02027 ,  C12N9/2497 ,  C12Q1/6844 ,  C12Q2521/531

Abstract: The present invention provides uracil-DNA glycosylase (UDG) gene originating from Psychrobacter sp. HJ147, and amino acid sequences deduced from the gene; expression and purification of Psp HJ147 UDG gene in Escherichia coli; and characterization of UDG obtained therefrom, and the use thereof in a polymerase chain reaction (PCR). The UDG according to the present invention has a specific activity of excising uracil bases in a uracil-containing DNA substrates at a low temperature, and is easily heat-inactivated. It thus can effectively eliminate cross contamination and carry-over contamination of PCR templates often occurring after a PCR process using dUTP. Therefore, it is useful for increasing preciseness (elimination of false positives), purity and amplification efficiency of PCR.

Abstract translation: 本发明提供源自Psychrobacter sp。的尿嘧啶-DNA糖基化酶（UDG）基因。 HJ147和从该基因推导出的氨基酸序列; 在大肠杆菌中表达和纯化Psp HJ147 UDG基因; 和由其获得的UDG的表征，以及其在聚合酶链式反应（PCR）中的用途。 根据本发明的UDG在低温下具有在含尿嘧啶DNA基质中的尿嘧啶碱基的比活性，并且容易热灭活。 因此，可以有效地消除在使用dUTP的PCR过程之后经常发生的PCR模板的交叉污染和携带污染。 因此，提高PCR的精确度（消除假阳性），纯度和扩增效率是有用的。

公开(公告)号：US20100118325A1

公开(公告)日：2010-05-13

申请号：US12481743

申请日：2009-06-10

Applicant: Chang-hwan KIM ,  Gun-a KIM

Inventor： Chang-hwan KIM ,  Gun-a KIM

IPC: G06F3/12

CPC classification number: G06K15/00 ,  G06F3/1212 ,  G06F3/1237 ,  G06F3/124 ,  G06F3/1279 ,  H04N1/00127 ,  H04N1/0083 ,  H04N1/00912 ,  H04N1/00931 ,  H04N1/32

Abstract: An image forming apparatus includes a communication interface to receive a job, and a control board including a plurality of dedicated processors to support their respective functions and a serial interface connected each of the plurality of dedicated processors. The control board controls one of the plurality of dedicated processors corresponding to a received job to perform the received job. Accordingly, the functions of the image forming apparatus are effectively operated and may be performed in parallel with one another.

Abstract translation: 图像形成装置包括用于接收作业的通信接口以及包括多个专用处理器以支持其各自功能的控制板和连接多个专用处理器中的每一个的串行接口。 控制板控制与所接收的作业相对应的多个专用处理器中的一个，以执行所接收的作业。 因此，图像形成装置的功能被有效地操作并且可以彼此并行地执行。

公开(公告)号：US20080299609A1

公开(公告)日：2008-12-04

申请号：US11823123

申请日：2007-06-26

Applicant: Suk-Tae Kwon ,  Mi-Sun Lee ,  Gun-A. Kim ,  Jung-Hyun Lee

Inventor： Suk-Tae Kwon ,  Mi-Sun Lee ,  Gun-A. Kim ,  Jung-Hyun Lee

IPC: C12P21/04 ,  C12N9/24 ,  C12N15/11 ,  C12N9/00 ,  C12P19/34 ,  C12N15/00 ,  C12N1/20

CPC classification number: C12Y302/02027 ,  C12N9/2497 ,  C12Q1/6844 ,  C12Q2521/531

Abstract: The present invention provides uracil-DNA glycosylase (UDG) gene originating from Psychrobacter sp. HJ147, and amino acid sequences deduced from the gene; expression and purification of Psp HJ147 UDG gene in Escherichia coli; and characterization of UDG obtained therefrom, and the use thereof in a polymerase chain reaction (PCR). The UDG according to the present invention has a specific activity of excising uracil bases in a uracil-containing DNA substrates at a low temperature, and is easily heat-inactivated. It thus can effectively eliminate cross contamination and carry-over contamination of PCR templates often occurring after a PCR process using dUTP. Therefore, it is useful for increasing preciseness (elimination of false positives), purity and amplification efficiency of PCR.

Abstract translation: 本发明提供源自Psychrobacter sp。的尿嘧啶-DNA糖基化酶（UDG）基因。 HJ147和从该基因推导出的氨基酸序列; 在大肠杆菌中表达和纯化Psp HJ147 UDG基因; 和由其获得的UDG的表征，以及其在聚合酶链式反应（PCR）中的用途。 根据本发明的UDG在低温下具有在含尿嘧啶DNA基质中的尿嘧啶碱基的比活性，并且容易热灭活。 因此，可以有效地消除在使用dUTP的PCR过程之后经常发生的PCR模板的交叉污染和携带污染。 因此，提高PCR的精确度（消除假阳性），纯度和扩增效率是有用的。