发明授权
US07727740B1 Methods for determining whether a chemical agent modulates enzymatic activity of an L-2-hydroxy acid oxidase
失效
用于确定化学试剂是否调节L-2-羟基酸氧化酶的酶活性的方法
- 专利标题： Methods for determining whether a chemical agent modulates enzymatic activity of an L-2-hydroxy acid oxidase
- 专利标题（中）： 用于确定化学试剂是否调节L-2-羟基酸氧化酶的酶活性的方法
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申请号： US11490840申请日： 2006-07-21
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公开(公告)号： US07727740B1公开(公告)日： 2010-06-01
- 发明人: Pek Yee Lum , Rebecca Kaplan , Tian-Quan Cai
- 申请人： Pek Yee Lum , Rebecca Kaplan , Tian-Quan Cai
- 申请人地址： US NJ Rahway
- 专利权人： Merck Sharp & Dohme Corp.
- 当前专利权人： Merck Sharp & Dohme Corp.
- 当前专利权人地址： US NJ Rahway
- 代理机构： Christensen O'Connor Johnson Kindness PLLC
- 主分类号： C12Q1/26
- IPC分类号： C12Q1/26 ; A61K49/00
摘要：
The present invention provides methods for screening for one or more chemical agents that modulate the enzymatic activity of an L-2-hydroxy acid oxidase. The methods comprise the steps of (a) contacting an L-2-hydroxy acid oxidase, in a solution in vitro, with one or more chemical agents in the presence of a substrate that is capable of being oxidized by the L-2-hydroxy acid oxidase; (b) measuring the enzymatic activity of the L-2-hydroxy acid oxidase in the presence of the chemical agent to identify one or more candidate chemical agents that modulate L-2-hydroxy acid oxidase activity in vitro; and (c) administering the one or more identified candidate chemical agents to a test animal and measuring one or more physiological parameters.