发明授权
- 专利标题： IL-21 production in prokaryotic hosts
- 专利标题（中）： 原核生物中的IL-21产生
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申请号： US11539036申请日： 2006-10-05
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公开(公告)号： US07745176B2公开(公告)日： 2010-06-29
- 发明人: Bruce L. Zamost , Douglas C. Covert
- 申请人： Bruce L. Zamost , Douglas C. Covert
- 申请人地址： US WA Seattle
- 专利权人： ZymoGenetics, Inc.
- 当前专利权人： ZymoGenetics, Inc.
- 当前专利权人地址： US WA Seattle
- 代理商 Deborah A. Sawislak; Michelle L. Lewis
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12P21/02
摘要：
The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector.
公开/授权文献
- US20070048842A1 IL-21 PRODUCTION IN PROKARYOTIC HOSTS 公开/授权日：2007-03-01
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