A device for cell culture capable of automatically performing operations for cell culture over several days to several months while minimizing the risk of contamination. A new medicine can be supplied to an incubator means by using a medicine supply means or unnecessary wastewater can be discharged from the incubator means by using a wastewater discharge means without taking out the incubator means disposed in heat insulation box means from a heat insulation box, and the state of the cell culture can be observed with the incubator means formed in the heat insulation box means. Accordingly, the outside air does not enter directly into the incubator means during culturing, and the risk of contamination is completely eliminated. As a result, the culturing operations can be automatically performed over a long period.