The invention comprises methods of patterning a plurality of substrates, and imprint templates used in imprint lithography. In one implementation, a method of patterning a plurality of substrates includes providing an imprint template having a plurality of spaced features. A first substrate is imprinted with the imprint template effective to form a plurality of recesses into the first substrate from the spaced features. After imprinting the first substrate, an elevationally outermost portion of the spaced features is removed effective to reduce elevation of the spaced features. After the removing, a second substrate is imprinted with the imprint template using the elevation-reduced spaced features effective to form a plurality of recesses into the second substrate from the elevation-reduced spaced features. Other aspects and implementations are contemplated.