The method of activating a vehicle fuel cell for ensuring maximum performance, improves performance of a polymer electrolyte membrane fuel cell, reduces a hydrogen usage, and has stable performance after manufactured. The method includes: placing a fuel cell in an activation device so as to be activated; changing a humidification state of a humidifier which supplies vapor to the fuel cell and a state of a cooling water; supplying a reactant gas to the fuel cell and maintaining a no-load state; maintaining a load state while changing a flow rate of the vapor and gas supplied to the fuel cell; changing the sate of the fuel cell to the no-load state, and re-supplying the reactant gas to the fuel cell; and comparing data measured when the fuel cell operates in the no-load state and data measured when the fuel cell operates in the load state respectively with a reference value.