Provided is a flux regulation method for use in a power converter, wherein the method is carried out by a flux bias controller. The flux bias controller includes a current detector which is configured to detect the primary current of the transformer of the power converter in a predetermined switching cycle, a DC bias processor which is configured to obtain the duty ratio control signal according to the sampled primary current outputted from the current detector for a switching cycle later than the predetermined switching cycle, and a PWM controller which is configured to generate driving signals to control the on/off operations of the switching circuit of the power converter according to the duty ratio control signal, thereby suppressing the DC bias of the transformer by regulating the duty ratio of the switch circuit.