发明授权
US07820423B2 Method and a system for predicting protein functional site, a method for improving protein function, and a function-modified protein
有权
用于预测蛋白质功能位点的方法和系统,改善蛋白质功能的方法和功能修饰的蛋白质
- 专利标题: Method and a system for predicting protein functional site, a method for improving protein function, and a function-modified protein
- 专利标题(中): 用于预测蛋白质功能位点的方法和系统,改善蛋白质功能的方法和功能修饰的蛋白质
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申请号: US11806448申请日: 2007-05-31
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公开(公告)号: US07820423B2公开(公告)日: 2010-10-26
- 发明人: Hirofumi Doi , Hideaki Hiraki , Akio Kanai
- 申请人: Hirofumi Doi , Hideaki Hiraki , Akio Kanai
- 申请人地址: JP Saitama
- 专利权人: Japan Science And Technology Corporation
- 当前专利权人: Japan Science And Technology Corporation
- 当前专利权人地址: JP Saitama
- 代理机构: Wenderoth, Lind & Ponack, L.L.P
- 优先权: JP1997-019248 19970131; JP1997-019249 19970131; JP1997-332100 19971202; JP1998-018699 19980130
- 主分类号: C12N9/12
- IPC分类号: C12N9/12
摘要:
The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known. More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present also provides a system for predicting a functional site for automatically performing said methods. Additionally, the present application provides a method for preparing a function-modified protein comprising subjecting the amino-acid residues composing the functional site identified by the method described above to artificial mutation, and a novel thermophilic DNA polymerase prepared by the method.