公开(公告)号：US07231301B2

公开(公告)日：2007-06-12

申请号：US10345205

申请日：2003-01-16

申请人： Hirofumi Doi ,  Hideaki Hiraki ,  Akio Kanai

发明人： Hirofumi Doi ,  Hideaki Hiraki ,  Akio Kanai

IPC分类号： G06F17/11 ,  G06F17/50 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53

CPC分类号： C12N15/10 ,  C12N9/1252 ,  C12N15/102 ,  Y02A90/22

摘要： The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known. More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present also provides a system for predicting a functional site for automatically performing said methods. Additionally, the present application provides a method for preparing a function-modified protein comprising subjecting the amino-acid residues composing the functional site identified by the method described above to artificial mutation, and a novel thermophilic DNA polymerase prepared by the method.

摘要翻译： 本申请提供了使用其基因组数据或cDNA数据已知的生物体的全部蛋白质的数据来预测蛋白质的功能位点的方法。 更具体地，本申请提供了一种用于预测蛋白质功能位点的方法，包括以下步骤：计算整个蛋白质中寡肽的发生频率，计算作为发生频率的每个氨基酸残基的值作为发生频率 功能的代表性值，并通过使用功能的代表值作为指标预测蛋白质功能位点。 本发明还提供一种用于预测用于自动执行所述方法的功能部位的系统。 此外，本申请提供了制备功能修饰蛋白质的方法，包括将构成通过上述方法鉴定的功能位点的氨基酸残基进行人工突变，以及通过该方法制备的新型嗜热DNA聚合酶。

公开(公告)号：US07820423B2

公开(公告)日：2010-10-26

申请号：US11806448

申请日：2007-05-31

申请人： Hirofumi Doi ,  Hideaki Hiraki ,  Akio Kanai

发明人： Hirofumi Doi ,  Hideaki Hiraki ,  Akio Kanai

IPC分类号： C12N9/12

CPC分类号： C12N15/10 ,  C12N9/1252 ,  C12N15/102 ,  Y02A90/22

摘要： The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known. More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present also provides a system for predicting a functional site for automatically performing said methods. Additionally, the present application provides a method for preparing a function-modified protein comprising subjecting the amino-acid residues composing the functional site identified by the method described above to artificial mutation, and a novel thermophilic DNA polymerase prepared by the method.

摘要翻译： 本申请提供了使用其基因组数据或cDNA数据已知的生物体的全部蛋白质的数据来预测蛋白质的功能位点的方法。 更具体地，本申请提供了一种用于预测蛋白质功能位点的方法，包括以下步骤：计算整个蛋白质中寡肽的发生频率，计算作为发生频率的每个氨基酸残基的值作为发生频率 功能的代表性值，并通过使用功能的代表值作为指标预测蛋白质功能位点。 本发明还提供一种用于预测用于自动执行所述方法的功能部位的系统。 此外，本申请提供了制备功能修饰蛋白质的方法，包括将构成通过上述方法鉴定的功能位点的氨基酸残基进行人工突变，以及通过该方法制备的新型嗜热DNA聚合酶。

公开(公告)号：US20080262201A1

公开(公告)日：2008-10-23

申请号：US11806448

申请日：2007-05-31

申请人： Hirofumi Doi ,  Hideaki Hiraki ,  Akio Kanai

发明人： Hirofumi Doi ,  Hideaki Hiraki ,  Akio Kanai

IPC分类号： C07K1/00 ,  G01N33/50

CPC分类号： C12N15/10 ,  C12N9/1252 ,  C12N15/102 ,  Y02A90/22

摘要： The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known. More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present also provides a system for predicting a functional site for automatically performing said methods. Additionally, the present application provides a method for preparing a function-modified protein comprising subjecting the amino-acid residues composing the functional site identified by the method described above to artificial mutation, and a novel thermophilic DNA polymerase prepared by the method.

摘要翻译： 本申请提供了使用其基因组数据或cDNA数据已知的生物体的全部蛋白质的数据来预测蛋白质的功能位点的方法。 更具体地，本申请提供了一种用于预测蛋白质功能位点的方法，包括以下步骤：计算整个蛋白质中寡肽的发生频率，计算作为发生频率的每个氨基酸残基的值作为发生频率 功能的代表性值，并通过使用功能的代表值作为指标预测蛋白质功能位点。 本发明还提供一种用于预测用于自动执行所述方法的功能部位的系统。 此外，本申请提供了制备功能修饰蛋白质的方法，包括将构成通过上述方法鉴定的功能位点的氨基酸残基进行人工突变，以及通过该方法制备的新型嗜热DNA聚合酶。

公开(公告)号：US08003346B2

公开(公告)日：2011-08-23

申请号：US11994437

申请日：2006-07-04

申请人： Tadaaki Tokida ,  Satoshi Hihara ,  Takashi Kudou ,  Akira Kawamura ,  Hirofumi Doi ,  Yoshizumi Ishino

发明人： Tadaaki Tokida ,  Satoshi Hihara ,  Takashi Kudou ,  Akira Kawamura ,  Hirofumi Doi ,  Yoshizumi Ishino

IPC分类号： G01N33/52 ,  C07H17/00 ,  C07K14/00

CPC分类号： C07K14/4738 ,  C12N9/1252 ,  C12N15/69

摘要： The present invention is to construct a DNA replication reaction system which is excellent in versatility and is easily used. An amino acid sequence of a PCNA monomer which is one of factors involved in DNA replication is prepared so that amino acid residues causing mutual charge repulsion constitute a site which causes, when an N terminal region of the PCNA monomer and a C terminal region of another PCNA monomer act as an interface to form a multimeric complex, an intermolecular interaction of the monomers in an interface region of the monomers.

摘要翻译： 本发明是构建通用性优异且易于使用的DNA复制反应体系。 制备作为涉及DNA复制的因素之一的PCNA单体的氨基酸序列，使得导致相互电荷排斥的氨基酸残基构成了当PCNA单体的N末端区域和另一个的C末端区域时引起的位点 PCNA单体作为形成多聚体复合物的界面，单体在界面区域中的分子间相互作用。

公开(公告)号：US20110183331A1

公开(公告)日：2011-07-28

申请号：US12993338

申请日：2009-05-19

申请人： Hirofumi Doi ,  Masahiro Matsuoka ,  Tatsuhiro Nakano ,  Chizuru Nagayoshi

发明人： Hirofumi Doi ,  Masahiro Matsuoka ,  Tatsuhiro Nakano ,  Chizuru Nagayoshi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6841 ,  C12Q2543/10 ,  C12Q2527/125 ,  C12Q2545/101

摘要： Disclosed is a method for identifying the presence of a target gene mRNA, which comprises hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes, wherein the oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA, and wherein the dummy oligonucleic acids are substantially equal in length with the oligonucleic acid probes, and are neither hybridizable with regions of the target gene mRNA with which the oligonucleic acid probes hybridize nor with the oligonucleic acid probes.

摘要翻译： 公开了一种鉴定目标基因mRNA的存在的方法，其包括将一种或多种寡核酸探针与组织样品中表达的靶基因mRNA杂交，并检测添加到以下的至少一种的低分子量化合物标记： 寡核酸探针的碱基，其中寡聚核酸探针与组织样品接触，用于与组织样品预处理（预杂交）（预杂交）一种或多种假寡聚核酸或寡核酸探针的混合物与靶基因mRNA杂交 使虚拟寡核酸与样品组织接触，将寡核酸探针与靶基因mRNA杂交，并且其中虚拟寡核酸与寡核酸探针的长度基本相等，并且不与靶基因的区域杂交 寡聚核酸探针与其杂交的mRNA也不与低聚核酸探针杂交。

公开(公告)号：US20100231070A1

公开(公告)日：2010-09-16

申请号：US12305872

申请日：2007-04-19

申请人： Kenta Hatano ,  Hirofumi Doi ,  Toshihiko Miyake

发明人： Kenta Hatano ,  Hirofumi Doi ,  Toshihiko Miyake

IPC分类号： H02K7/06

CPC分类号： H02K7/06

摘要： An actuator having an output axis which is moved linearly by a rotatory force, and a boss for holding the above-mentioned output axis in such a way that the output axis can move linearly includes a connecting member for propagating the linear motion of the above-mentioned output axis to an external device. The above-mentioned connecting member is comprised of a joint connected to the above-mentioned output axis, and a link plate having an end rotatably connected to the above-mentioned joint, and another end rotatably connected to the external device. A mechanism for preventing rotation of the above-mentioned output axis is constructed of only a connecting portion for connecting the above-mentioned joint, the above-mentioned link plate, and the above-mentioned external device to one another.

摘要翻译： 具有通过旋转力线性移动的输出轴的致动器和用于保持上述输出轴线的凸起，使得输出轴线性线性地移动，具有用于传播上述输出轴的直线运动的连接部件， 提到的输出轴到外部设备。 上述连接构件包括连接到上述输出轴线的接头和具有可旋转地连接到上述接头的端部的连接板，以及可旋转地连接到外部设备的另一端。 用于防止上述输出轴的旋转的机构仅由用于将上述接头，上述连接板和上述外部装置彼此连接的连接部分构成。

公开(公告)号：US20070134663A1

公开(公告)日：2007-06-14

申请号：US10568578

申请日：2004-08-13

申请人： Satoshi Hihara ,  Hirofumi Doi

发明人： Satoshi Hihara ,  Hirofumi Doi

IPC分类号： C12Q1/68 ,  C12Q1/48 ,  C12N9/99

CPC分类号： G01N33/6875 ,  C12Q1/485 ,  G01N2500/02

摘要： The task is to provide protein interaction inhibitors and/or detection method for interaction inhibitors related to regulation of T cell activation and production of IL-2. The present invention provides inhibitors of interaction between PKC theta and KPNA1, and inhibitors of interaction between KPNA1 and NF-kappaB. Inhibitors of interaction is defined as an candidate compound demonstrating the inhibition of interaction by assaying whether interaction has occurred or not between 2 types of protein and the candidate compound under the conditions allowing interaction of each combination. The detection kit is equipped with samples providing proteins of combinations to be interacted.

摘要翻译： 任务是提供蛋白质相互作用抑制剂和/或与调节T细胞活化和产生IL-2相关的相互作用抑制剂的检测方法。 本发明提供PKCθ与KPNA1之间相互作用的抑制剂，以及KPNA1与NF-κB之间相互作用的抑制剂。 相互作用的抑制剂被定义为通过测定在允许每种组合的相互作用的条件下，在两种类型的蛋白质和候选化合物之间是否发生相互作用来证明相互作用的抑制的候选化合物。 检测试剂盒配备提供待相互作用的组合蛋白质的样品。

公开(公告)号：US07053510B2

公开(公告)日：2006-05-30

申请号：US10118904

申请日：2002-04-10

申请人： Hirofumi Doi

发明人： Hirofumi Doi

IPC分类号： H02K5/00

CPC分类号： H02K5/00

摘要： An electrical actuator has a DC motor which is provided with a rotor having a permanent magnet for forming a plurality of magnetic poles around a periphery thereof and a stator having a plurality of energizing coils and being disposed around a periphery of the rotor to thereby generate a rotary force for the rotor. In mounting this DC motor on a heat-generating member which generates radiant heat, a member for shielding radiant heat from a heat-generating member is disposed between the DC motor and the heat generating member. In this manner, the radiant heat from the heat-generating member is prevented from reaching the DC motor, whereby the problems in the DC motor associated with the radiant heat can be prevented.

公开(公告)号：US06571469B2

公开(公告)日：2003-06-03

申请号：US09844245

申请日：2001-04-26

申请人： Masanobu Okada ,  Kazuyoshi Nakaya ,  Hiroyuki Nakaji ,  Hirofumi Doi ,  Iku Nagai ,  Junichi Nakasone

发明人： Masanobu Okada ,  Kazuyoshi Nakaya ,  Hiroyuki Nakaji ,  Hirofumi Doi ,  Iku Nagai ,  Junichi Nakasone

IPC分类号： H01K310

CPC分类号： H01L23/49805 ,  H01L21/4853 ,  H01L2924/0002 ,  H01R43/0256 ,  H05K1/141 ,  H05K3/0052 ,  H05K3/3442 ,  H05K3/3478 ,  H05K3/368 ,  H05K3/403 ,  H05K3/42 ,  H05K2201/09181 ,  H05K2201/10984 ,  H05K2203/041 ,  H05K2203/043 ,  Y02P70/613 ,  Y10T29/49117 ,  Y10T29/49165 ,  Y10T29/49789 ,  Y10T29/4979 ,  H01L2924/00

摘要： A blanking plate is attached to the bottom surfaces of a split board and a remaining board, which are separated from one bare board. After depositing solder paste on a back electrode of the split board, a solder ball is attached on the solder paste. The split board is then heated to melt the solder ball. The molten solder flows along the back electrode and an edge electrode into a through-hole. A portion of the molten solder swells out of the bottom surface of the bare board, and solidifies and bonds to the back electrode and the edge electrode.

公开(公告)号：US06182057B2

公开(公告)日：2001-01-30

申请号：US08990050

申请日：1997-12-12

申请人： Yasuhiro Kikuchi ,  Hirofumi Doi

发明人： Yasuhiro Kikuchi ,  Hirofumi Doi

IPC分类号： G06F1518

CPC分类号： G06N3/126

摘要： A genetic algorithm executing device, in which search of a genetic algorithm is improved, sets a distribution of characters at each position of character strings represent candidates of solution, obtain the degree of disorder at each position by the distributions of characters, set a larger mutation rate at the position of which degree of disorder is higher. This device performs a mutation operation on the character strings according to the mutation rates. This device generates the new character strings by selecting positions of which degrees of disorder are higher than a predetermined value, and changing the character at the selected positions.

摘要翻译： 遗传算法执行装置，其中改进了遗传算法的搜索，设置了字符串每个位置处的字符分布表示解的候选，通过字符分布获得每个位置的无序程度，设置较大的突变 在无序程度较高的位置的比率。 该装置根据突变率对字符串进行突变操作。 该装置通过选择无序程度高于预定值的位置并改变所选位置的字符来生成新的字符串。