A method for calibration of a digital celestial sensor is disclosed. First, an integrated mathematic model for imaging of a celestial sensor is established according to external and internal parameters of the calibration system of the celestial sensor. Second, by rotating two axes of a rotator by different angles, calibration points data are acquired and sent to a processing computer through an interface circuit. Finally, a two-step calibration program is implemented to calculate the calibration parameters by substituting calibration points' data to the integrated mathematic model. An application device of the calibration method is also provided. The device may include a celestial simulator to provide simulated sunlight or starlight, a two-axis rotator to acquire different calibration points' data, and a processing computer to record the calibration points' data and calculate the calibration parameters.