发明授权
- 专利标题: High-pressure inclusion body solubilization and protease clipping of recombinant fusion proteins
- 专利标题(中): 重组融合蛋白的高压包涵体溶解和蛋白酶剪切
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申请号: US12098877申请日: 2008-04-07
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公开(公告)号: US07829681B2公开(公告)日: 2010-11-09
- 发明人: Matthew B. Seefeldt , Lyndal K. Hesterberg , Theodore W. Randolph , John F. Carpenter
- 申请人: Matthew B. Seefeldt , Lyndal K. Hesterberg , Theodore W. Randolph , John F. Carpenter
- 申请人地址: US CO Aurora
- 专利权人: Barofold Inc.
- 当前专利权人: Barofold Inc.
- 当前专利权人地址: US CO Aurora
- 代理机构: Cooley LLP
- 主分类号: C07K14/00
- IPC分类号: C07K14/00 ; C12N9/48 ; C12P21/04
摘要:
Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
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