发明授权
US07838303B2 Peptide derivatization method to increase fragmentation information from MS/MS spectra
有权
肽衍生化方法从MS / MS谱增加碎片信息
- 专利标题： Peptide derivatization method to increase fragmentation information from MS/MS spectra
- 专利标题（中）： 肽衍生化方法从MS / MS谱增加碎片信息
-
申请号： US11494383申请日： 2006-07-27
-
公开(公告)号： US07838303B2公开(公告)日： 2010-11-23
- 发明人: Patrick D. Perkins , Steven M. Fischer
- 申请人： Patrick D. Perkins , Steven M. Fischer
- 申请人地址： US CA Santa Clara
- 专利权人： Agilent Technologies, Inc.
- 当前专利权人： Agilent Technologies, Inc.
- 当前专利权人地址： US CA Santa Clara
- 主分类号： G01N24/00
- IPC分类号： G01N24/00
摘要：
A method of facilitating analysis of a peptide in a mass spectrometer comprising derivatizing the C-terminus of the peptide with an amino acid residue via a reaction with a carbodiimide reagent, yielding a derivative peptide, ionizing the derivative peptide with a double charge, and fragmenting the ionized derivative peptide in a mass spectrometry system, wherein binary fragments of the ionized derivative each include a charge, facilitating sequence analysis of the peptide.