Invention Grant
- Patent Title: Twin-arginine translocation in Bacillus
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Application No.: US11809528Application Date: 2007-06-01
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Publication No.: US07884191B2Publication Date: 2011-02-08
- Inventor: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
- Applicant: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
- Applicant Address: US CA Palo Alto
- Assignee: Danisco US Inc.
- Current Assignee: Danisco US Inc.
- Current Assignee Address: US CA Palo Alto
- Agency: Danisco US Inc. Morrison & Foerster LLP
- Agent Margaret A. Powers
- Main IPC: C07K14/32
- IPC: C07K14/32 ; C12N9/00 ; A61K39/00

Abstract:
Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
Public/Granted literature
- US20090093025A1 Twin-arginine translocation in Bacillus Public/Granted day:2009-04-09
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