A method and system for automatically focusing an image received by an image capturing unit (100) relative to a target is disclosed. The target is present in a sequence of one or more scanned frames. The method includes dividing (302) each frame from the sequence of one or more scanned frames in a plurality of sections. The method further includes iteratively determining (304) a relative focus of the target within each section of the plurality of sections. The method further includes adjustment (306) of a position of at least a first light path adjustment element in response to the determined relative focus.