- 专利标题: PCV2 immunogenic compositions and methods of producing such compositions
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申请号: US12138020申请日: 2008-06-12
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公开(公告)号: US07910306B2公开(公告)日: 2011-03-22
- 发明人: Marc Eichmeyer , Greg Nitzel , Merrill Schaeffer
- 申请人: Marc Eichmeyer , Greg Nitzel , Merrill Schaeffer
- 申请人地址: US MO Saint Joseph
- 专利权人: Boehringer Ingelheim Vetmedica, Inc.
- 当前专利权人: Boehringer Ingelheim Vetmedica, Inc.
- 当前专利权人地址: US MO Saint Joseph
- 代理商 Michael P. Morris; Julie A. Scott
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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