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    PCV2 immunogenic compositions and methods of producing such compositions
    4.
    发明授权
    PCV2 immunogenic compositions and methods of producing such compositions 有权
    PCV2免疫原性组合物及其制备方法

    公开(公告)号：US07829101B2

    公开(公告)日：2010-11-09

    申请号：US12137960

    申请日：2008-06-12

    申请人： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

    发明人： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

    IPC分类号： A61K39/12

    CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

    摘要： An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

    摘要翻译： 提供了一种用于从猪圆环病毒2型开放阅读框架2回收蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中所含的细胞的步骤，导致病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取程序。

    PCV2 immunogenic compositions and methods of producing such compositions
    10.
    发明授权
    PCV2 immunogenic compositions and methods of producing such compositions 有权
    PCV2免疫原性组合物及其制备方法

    公开(公告)号：US07700285B1

    公开(公告)日：2010-04-20

    申请号：US11319975

    申请日：2005-12-29

    申请人： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

    发明人： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

    IPC分类号： C12Q1/68

    CPC分类号： A61K39/12 ,  A61K2039/5258 ,  A61K2039/552 ,  A61K2039/55511 ,  A61K2039/55566 ,  C07K14/005 ,  C12N2750/10022 ,  C12N2750/10034

    摘要： An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

    摘要翻译： 提供了一种用于从猪圆环病毒2型开放阅读框架2回收蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中所含的细胞的步骤，使病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取方法。