A high voltage start-up circuit with constant current control applied to a switching mode power converter is provided. The high voltage start-up circuit includes a high voltage junction transistor, a control transistor, a current detecting resistor and a bias resistor. The drain of the junction transistor is connected to a high power supply, the gate of the junction transistor is connected to the drain of the control transistor, and the source of the junction transistor is connected to the current detecting resistor. The voltage drop crossing the current detecting resistor is kept constant to have the junction transistor output a constant current. The bias resistor which is connected between the gate of the junction transistor and the output end of the high voltage start-up circuit has the gate to source bias voltage of the junction transistor kept constant to output constant current.