A packet loss estimation technique is disclosed that utilizes the sampled flow level statistics that are routinely collected in operational networks, thereby obviating the need for any new router features or measurement infrastructure. The technique is specifically designed to handle the challenges of sampled flow-level aggregation such as information loss resulting from packet sampling, and generally comprises: receiving a first record of sampled packets for a flow from a first network element; receiving a second record of sampled packets for the flow from a second network element communicating with the first network element; correlating sampled packets from the flow at the first network element and the second network element to a measurement interval; and estimating the packet loss using a count of the sampled packets correlated to the measurement interval.