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发明授权
US07968695B2 Nucleic acids encoding recombinant 56 and 82 kDa antigens from gametocytes of Eimeria maxima and their uses 有权
编码重组56和82 kDa抗原的核酸从艾美球虫的配子体及其用途

Nucleic acids encoding recombinant 56 and 82 kDa antigens from gametocytes of Eimeria maxima and their uses
摘要：
The present invention provides the recombinant cloning and sequencing of two of the major Eimeria maxima gametocyte antigens having molecular weights of 56 and 82 kDa and the expression of these recombinant antigens in an E. coli expression system using the plasmid pTrcHis. The subject invention also provides a vaccine against coccidiosis comprising the recombinant 56 kDa or 82 kDa antigen. The subject invention also provides two 30 kDa proteins and three 14 kDa proteins from Eimeria maxima gametocytes having at the N-terminal end the amino acid sequence described herein. The subject invention also provides a vaccine against coccidiosis comprising the recombinant 56 kDa or 82 kDa antigen and any of the aforementioned proteins. Additionally, the subject invention also provides a method of immunizing a subject against infection by Eimeria tenella, Eimeria maxima, Eimeria acervulina, Eimeria necatrix, Eimeria praecox, Eimeria mitis or Eimeria brunetti, or a microorganism expressing an immunologically cross-reactive antigen, comprising the step of administering to the subject any of the aforementioned vaccines.
摘要（中）：

本发明提供了分子量为56和82kDa的两种主要的艾美球虫最大配子型抗原的重组克隆和测序，以及这些重组抗原在使用质粒pTrcHis的大肠杆菌表达系统中的表达。 本发明还提供了包含重组56kDa或82kDa抗原的针对球虫病的疫苗。 本发明还提供了在最终端具有本文所述氨基酸序列的来自艾美球虫最大配子配子的两个30kDa蛋白质和三个14kDa蛋白质。 本发明还提供了包含重组56kDa或82kDa抗原和任何上述蛋白质的抗球虫病疫苗。 此外，本发明还提供了免疫受试者免受艾美球虫艾美球虫，艾美球虫艾美球虫，艾美球虫，艾美球虫，艾美球虫或艾美球虫艾美球虫或表达免疫交叉反应性抗原的微生物的感染的方法， 向受试者施用任何上述疫苗的步骤。

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