发明授权
US07972855B2 Microorganisms that extracellularly secrete lipids and methods of producing lipid and lipid vesicles encapsulating lipids using said microorganisms
有权
细胞外分泌脂质的微生物和使用所述微生物包裹脂质的脂质和脂质囊泡的产生方法
- 专利标题: Microorganisms that extracellularly secrete lipids and methods of producing lipid and lipid vesicles encapsulating lipids using said microorganisms
- 专利标题(中): 细胞外分泌脂质的微生物和使用所述微生物包裹脂质的脂质和脂质囊泡的产生方法
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申请号: US11607032申请日: 2006-12-01
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公开(公告)号: US07972855B2公开(公告)日: 2011-07-05
- 发明人: Kengo Akimoto , Hiroshi Kawashima , Sakayu Shimizu
- 申请人: Kengo Akimoto , Hiroshi Kawashima , Sakayu Shimizu
- 申请人地址: JP Osaka-shi, Osaka
- 专利权人: Suntory Holdings Limited
- 当前专利权人: Suntory Holdings Limited
- 当前专利权人地址: JP Osaka-shi, Osaka
- 代理机构: Drinker Biddle & Reath LLP
- 优先权: JP11-229509 19990813
- 主分类号: C12N15/04
- IPC分类号: C12N15/04 ; C12N15/01 ; C12N1/15 ; C12N1/13 ; C12P1/00 ; C12P7/64 ; C12P1/02
摘要:
There are provided microorganisms, for example, Mortierella alpina, having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.