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98 条记录 (耗时 0.024 秒)

    Method of preparing 2-deoxyribose 5-phosphate
    2.
    发明授权
    Method of preparing 2-deoxyribose 5-phosphate 有权
    2-脱氧核糖5-磷酸的制备方法

    公开(公告)号：US07927843B2

    公开(公告)日：2011-04-19

    申请号：US11878558

    申请日：2007-07-25

    申请人： Sakayu Shimizu ,  Jun Ogawa

    发明人： Sakayu Shimizu ,  Jun Ogawa

    IPC分类号： C12P19/02

    CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

    摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphates or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphates or the enzymes derived from the microorganism.

    摘要翻译： 本发明公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶但基本上不含磷酸盐的微生物本身存在下使甘油醛-3-磷酸和乙醛反应制备2-脱氧核糖-5-磷酸的方法， 微生物。 本发明还公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶和丙糖磷酸异构酶但基本上不含磷酸盐的微生物本身存在下使磷酸二羟丙酮和乙醛反应制备2-脱氧核糖-5-磷酸的方法，或者 源于微生物的酶。

    Method of preparing 2-deoxyribose 5-phosphate
    5.
    发明申请
    Method of preparing 2-deoxyribose 5-phosphate 有权

    公开(公告)号：US20070298468A1

    公开(公告)日：2007-12-27

    申请号：US11878558

    申请日：2007-07-25

    申请人： Sakayu Shimizu ,  Jun Ogawa

    发明人： Sakayu Shimizu ,  Jun Ogawa

    IPC分类号： C12P19/02

    CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

    摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphatase or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphatase or the enzymes derived from the microorganism.

    High production method of prenyl alcohol using saccharomyces cells
    6.
    发明授权
    High production method of prenyl alcohol using saccharomyces cells 失效
    使用酵母细胞制备异戊醇的高生产方法

    公开(公告)号：US06974685B2

    公开(公告)日：2005-12-13

    申请号：US10022434

    申请日：2001-12-20

    申请人： Masayoshi Muramatsu ,  Shusei Obata ,  Sakayu Shimizu

    发明人： Masayoshi Muramatsu ,  Shusei Obata ,  Sakayu Shimizu

    IPC分类号： C12N1/38 ,  C12P7/04 ,  C12R1/645 ,  C12P7/02

    CPC分类号： C12N1/38 ,  C12P7/04

    摘要： The present invention provides a high production method of prenyl alcohol, which comprises culturing prenyl alcohol-producing cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene to produce and accumulate prenyl alcohol in the cells; allowing the accumulated prenyl alcohol to be secreted from the cells; and then collecting prenyl alcohol. The present invention enables prenyl alcohol to be highly produced in and effectively secreted from prenyl alcohol-producing cells by culturing the cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene.

    摘要翻译： 本发明提供了高产的异戊醇的制备方法，其包括在选自表面活性剂，脂肪或油中的至少一种的存在下，在具有增加的糖含量的培养基中培养异戊醇产生细胞， 和萜烯在细胞中产生和积累异戊醇; 允许从细胞中分泌积累的异戊醇; 然后收集异戊醇。 本发明通过在选自表面活性剂，表面活性剂，表面活性剂的至少一种的存在下，在具有增加的糖含量的介质中培养细胞，使异戊烯醇高产生并由异戊烯醇产生细胞有效分泌 脂肪或油，和萜烯。

    Method for production of geranylgeraniol and analogous compounds thereof by microorganisms
    7.
    发明授权
    Method for production of geranylgeraniol and analogous compounds thereof by microorganisms 失效
    通过微生物生产香叶基香叶醇及其类似物的方法

    公开(公告)号：US06972191B2

    公开(公告)日：2005-12-06

    申请号：US10022695

    申请日：2001-12-20

    申请人： Masayoshi Muramatsu ,  Shusei Obata ,  Sakayu Shimizu

    发明人： Masayoshi Muramatsu ,  Shusei Obata ,  Sakayu Shimizu

    IPC分类号： C07C33/02 ,  C12P7/04 ,  C12R1/85 ,  C12P7/02

    CPC分类号： C12P7/04

    摘要： The present invention provides a method for producing geranylgeraniol and analogous compounds thereof, which comprises culturing yeast cells (ascomycetes and deuteromycetes), bacterial cells, actinomycete cells or filamentous fungus cells, all of which are capable of producing geranylgeraniol and analogous compounds thereof, in a medium to produce and accumulate geranylgeraniol and analogous compounds thereof in the cells and/or in the extracellular environment; and then collecting these compounds. The present invention enables inexpensive mass production of geranylgeraniol and analogous compounds thereof by using microorganisms capable of producing geranylgeraniol, farnesol and/or nerolidol useful as biosynthetic intermediates of terpenes, carotenoids and/or steroids.

    摘要翻译： 本发明提供了一种生产香叶基香叶醇及其类似化合物的方法，其包括培养酵母细胞（子囊菌和氘核菌素），细菌细胞，放线菌细胞或丝状真菌细胞，所有这些能够产生香叶基香叶醇及其类似物 培养基以在细胞和/或细胞外环境中产生和积累香叶基香叶醇及其类似物; 然后收集这些化合物。 本发明可以通过使用能够产生香叶基香叶醇，法呢醇和/或橙花叔醇作为萜烯，类胡萝卜素和/或类固醇的生物合成中间体的微生物，从而廉价地批量生产香叶基香叶醇及其类似物。

    Microbial production of actinol
    9.
    发明授权
    Microbial production of actinol 有权
    微生物生成的肌动蛋白

    公开(公告)号：US06444460B1

    公开(公告)日：2002-09-03

    申请号：US09375129

    申请日：1999-08-16

    申请人： Sakayu Shimizu ,  Masaru Wada

    发明人： Sakayu Shimizu ,  Masaru Wada

    IPC分类号： C12P726

    CPC分类号： C12R1/06 ,  C12P7/26 ,  C12P41/002 ,  C12R1/01 ,  C12R1/15

    摘要： A process for making (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone by contacting (6R)-2,2,6-trimethylcyclohexanedione with a microorganism which is selected from microorganisms of the genera Cellulomonas, Corynebacterium, Planococcus and Arthrobacter and which is capable of the selective asymmetric reduction of (6R)-2,2,6-trimethylcyclohexanedione to (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone, and recovering the resulting (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone from the reaction mixture. The selective asymmetric reduction can be effected in the presence of a co-factor, such as, nicotinamide adenine dinucleotide (NAD), nicotinamide adenine dinucleotide phosphate (NADP), or said co-factor with glucose and glucose dehydrogenase (GDH), and/or in the presence of a surfactant. The product is useful for the synthesis of carotenoids, such as, zeaxanthin.

    摘要翻译： 通过使（6R）-2,2,6-三甲基环己二酮与微生物接触，制备（4R，6R）-4-羟基-2,2,6-三甲基环己酮的方法，所述微生物选自Cellulomonas，Corynebacterium，Planococcus 并且能够（6R）-2,2,6-三甲基环己二酮选择性不对称还原成（4R，6R）-4-羟基-2,2,6-三甲基环己酮，并回收得到的（4R，6R ）-4-羟基-2,2,6-三甲基环己酮。 选择性不对称还原可以在辅因子存在下进行，例如烟酰胺腺嘌呤二核苷酸（NAD），烟酰胺腺嘌呤二核苷酸磷酸（NADP）或与葡萄糖和葡萄糖脱氢酶（GDH）的所述辅因子，和/ 或在表面活性剂存在下。 该产品可用于合成类胡萝卜素，如玉米黄质。

    D-pantolactone hydrolase and gene encoding the same
    10.
    发明授权
    D-pantolactone hydrolase and gene encoding the same 失效
    D-泛酸内酯水解酶和编码基因的基因

    公开(公告)号：US06406898B1

    公开(公告)日：2002-06-18

    申请号：US08836047

    申请日：1997-09-19

    申请人： Keiji Sakamoto ,  Hideaki Yamada ,  Sakayu Shimizu ,  Michihiko Kobayashi

    发明人： Keiji Sakamoto ,  Hideaki Yamada ,  Sakayu Shimizu ,  Michihiko Kobayashi

    IPC分类号： C12N914

    CPC分类号： C12N9/18 ,  C12P17/04 ,  C12P41/001 ,  C12P41/005

    摘要： A novel enzyme which is useful in the optical resolution of D,L-pantolactone via D-selective asymmetric hydrolysis and a gene encoding the the same are provided. The invention discloses the gene coding for a natural D-pantolactone hydrolase (for example, one originating in Fusarium oxysporum) or proteins having an activity substantially equivalent thereto; host cells transformed with DNA containing a nucleotide sequence coding for said protein, processes for producing said protein via using said host cells and uses of said proteins and host cells.

    摘要翻译： 提供了可用于通过D-选择性不对称水解的D，L-泛酸内酯的光学拆分和编码其的基因的新型酶。 本发明公开了编码天然D-泛酸内酯水解酶（例如源于尖孢镰刀菌的一种）的基因或具有与其基本相当的活性的蛋白质; 用含有编码所述蛋白质的核苷酸序列的DNA转化的宿主细胞，通过使用所述宿主细胞产生所述蛋白质的过程和所述蛋白质和宿主细胞的用途。