A display substrate includes a base substrate, a conductive line on the base substrate, a switching element and a testing member. The switching element includes a first electrode formed on the semiconductor layer pattern and electrically connected to the conductive line, and a second electrode spaced apart from the first electrode and semiconductor layer pattern. The testing member includes a conductive line testing portion that is formed from the same layer as the conductive line and an electrode testing portion that is formed from the same layer as the first electrode. The conductive line testing portion and the electrode testing portion have substantially the same width as the conductive line and the first electrode, respectively. The testing member also includes a semiconductor layer testing portion. The display substrate lends itself to efficient manufacturing with reduced process time and cost.