A positioning and clamping apparatus, capable of positioning and clamping a panel material and removing the panel material from a locating pin under a state of releasing the clamping, is provided. A base having a contact surface contacting with the panel material is provided with the locating pin that penetrates through a through-hole of the panel material. A clamp arm moving between an escaping position and a clamping position is provided in a slit, and the clamp arm is moved between these positions by a reciprocating cam. When the clamp arm reaches the clamping position, the panel material is clamped by a clamp portion which is a tip of the clamp arm. A pusher driven by a pushing piston is provided inside the base, and the panel material is removed from the locating pin by the pusher.