发明授权
- 专利标题： Hydroxylase gene and use thereof
- 专利标题（中）： 羟化酶基因及其用途
-
申请号： US12302876申请日： 2007-05-18
-
公开(公告)号： US08148119B2公开(公告)日： 2012-04-03
- 发明人: Yoshikazu Fujii , Tomohiro Tamura
- 申请人： Yoshikazu Fujii , Tomohiro Tamura
- 申请人地址： JP Tokyo JP Tokyo
- 专利权人： Microbiopharm Japan Co., Ltd.,National Institute of Advanced Industrial Science and Technology
- 当前专利权人： Microbiopharm Japan Co., Ltd.,National Institute of Advanced Industrial Science and Technology
- 当前专利权人地址： JP Tokyo JP Tokyo
- 代理机构： Sughrue Mion, PLLC
- 优先权： JP2006-150843 20060531
- 国际申请： PCT/JP2007/060254 WO 20070518
- 国际公布： WO2007/138894 WO 20071206
- 主分类号： C12P15/00
- IPC分类号： C12P15/00 ; C12N1/20 ; C07H21/04
摘要：
A vitamin D3 hydroxylase is purified from Pseudonocardia autotrophica cell, and a primer is designed based on amino acid sequence obtained from hydroxylase. Subsequently, PCR is conducted using genomic DNA of Pseudonocardia autotrophica as a template to clone a gene for the vitamin D3 hydroxylase. By conducting a conversion reaction using a microorganism in which the vitamin D3 hydroxylase gene is expressed using a proper expression system, a hydroxide of vitamin D or the like (e.g., hydroxy vitamin D3) can be produced with high efficiency.
公开/授权文献
- US20100297712A1 HYDROXYLASE GENE AND USE THEREOF 公开/授权日：2010-11-25
信息查询
