Invention Grant
US08148137B2 Mutant microorganism having improved production ability of branched amino acid and method for preparing branched amino acid using the same
有权
具有提高支链氨基酸生产能力的突变体微生物及使用其制备支链氨基酸的方法
- Patent Title: Mutant microorganism having improved production ability of branched amino acid and method for preparing branched amino acid using the same
- Patent Title (中): 具有提高支链氨基酸生产能力的突变体微生物及使用其制备支链氨基酸的方法
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Application No.: US12097099Application Date: 2007-03-14
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Publication No.: US08148137B2Publication Date: 2012-04-03
- Inventor: Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
- Applicant: Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
- Applicant Address: KR Daejeon
- Assignee: Korea Advanced Institute of Science & Technology
- Current Assignee: Korea Advanced Institute of Science & Technology
- Current Assignee Address: KR Daejeon
- Agency: Merchant & Gould P.C.
- Priority: KR10-2007-0005268 20070117
- International Application: PCT/KR2007/001263 WO 20070314
- International Announcement: WO2008/088104 WO 20080724
- Main IPC: C12N1/21
- IPC: C12N1/21 ; C12P13/08

Abstract:
The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
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