发明授权
US08168418B2 Expression of HIV polypeptides and production of virus-like particles
有权
HIV多肽的表达和病毒样颗粒的产生
- 专利标题： Expression of HIV polypeptides and production of virus-like particles
- 专利标题（中）： HIV多肽的表达和病毒样颗粒的产生
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申请号： US12770905申请日： 2010-04-30
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公开(公告)号： US08168418B2公开(公告)日： 2012-05-01
- 发明人: Susan W Barnett , Jan Zur Megede , Indresh Srivastava , Ying Lian , Karin Hartog , Hong Liu , Catherine Greer , Mark Selby , Christopher Walker
- 申请人： Susan W Barnett , Jan Zur Megede , Indresh Srivastava , Ying Lian , Karin Hartog , Hong Liu , Catherine Greer , Mark Selby , Christopher Walker
- 代理商 Regina Bautista; Helen Lee
- 主分类号： C12N7/00
- IPC分类号： C12N7/00 ; A61K39/21
摘要：
The present invention relates to the efficient expression of HIV polypeptides in a variety of cell types, including, but not limited to, mammalian, insect, and plant cells. Synthetic expression cassettes encoding the HIV Gag-containing polypeptides are described, as are uses of the expression cassettes in applications including DNA immunization, generation of packaging cell lines, and production of Env-, tat- or Gag-containing proteins. The invention provides methods of producing Virus-Like Particles (VLPs), as well as, uses of the VLPs including, but not limited to, vehicles for the presentation of antigens and stimulation of immune response in subjects to whom the VLPs are administered.