Disclosed herein is an echo cancellation and echo channel estimation system that can relay transmit signals without interference with echo signals by canceling the undesired echo signals received by a relay. The echo cancellation and echo channel estimation system is designed to relay signals between a transmitter and a receiver through a relay using multiple antennas. The echo cancellation and echo channel estimation system includes a receive antenna, a preprocessing vector generation module, an echo cancellation module, and a transmit array antenna. The receive antenna receives a transmit signal from the transmitter and an echo signal via an echo channel. The preprocessing vector generation module generates a preprocessing vector and applies the preprocessing vector, the transmit signal and the echo signal, received from the receive antenna, to the echo cancellation module. The echo cancellation module cancels only the echo signal by multiplying the preprocessing vector, the transmit signal, and the echo signal. The transmit array antenna receives a retransmit signal from the echo cancellation module and resends the retransmit signal to the receiver.