A bio-sample image pickup device includes a light source module, a carrier, an image pickup unit, a first filter set, and a second filter set. The carrier carries the light source module and the bio-sample, and moves between a first position and a second position. The first filter set between the light source module and the image pickup unit for filtering the light emitted by the light source module, and the image pickup unit picks up the image of the bio-sample through the first filter set in the first position. The second filter set in the second position filters the light emitted by the light source module for allowing an operator to see the bio-sample in the second position through the second filter.