A fabrication method of a brightness enhancement film (BEF) including the following steps is provided. A light transmissive substrate is provided and has a first surface and a second surface opposite to the first surface. Then, a plurality of first rod-shaped lenses are formed on the first surface. The rod-shaped lenses extend along a first direction and are arranged along a second direction. After that, a plurality of second stripe-shaped prisms are formed on the second surface. The stripe-shaped prisms extend along the second direction and are arranged along the first direction. Next, an electromagnetic wave beam is made to pass through the rod-shaped lenses, the light transmissive substrate and the stripe-shaped prisms in sequence. A first portion of each of the stripe-shaped prisms exposes and leaves a second portion of each of the stripe-shaped prisms unexposed. Then, the second portions of the stripe-shaped prisms are removed.