发明授权
- 专利标题: Microfluidic system and method for sorting cell clusters and for the continuous encapsulation thereof following sorting thereof
- 专利标题(中): 用于分选细胞簇的微流体系统和方法以及在分选细胞簇后的连续包封
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申请号: US12437901申请日: 2009-05-08
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公开(公告)号: US08263023B2公开(公告)日: 2012-09-11
- 发明人: Sophie Le Vot , Jean Berthier , Florence Rivera
- 申请人: Sophie Le Vot , Jean Berthier , Florence Rivera
- 申请人地址: FR Paris
- 专利权人: Commissariat a l'Energie Atomique
- 当前专利权人: Commissariat a l'Energie Atomique
- 当前专利权人地址: FR Paris
- 代理机构: Alston & Bird LLP
- 优先权: FR0802575 20080513
- 主分类号: G01N15/06
- IPC分类号: G01N15/06 ; G01N33/00 ; G01N33/48
摘要:
A microfluidic system and method for sorting cell clusters, and for the continuous and automated encapsulation of the clusters, once sorted, in capsules of sizes suitable for those of these sorted clusters is provided. The microfluidic system comprises a substrate in which a microchannel array comprising a cell sorting unit is etched and around which a protective cover is bonded, and the sorting unit comprises deflection means capable of separating, during the flow thereof, relatively noncohesive cell clusters, each of size ranging from 20 μm to 500 μm and of 20 to 10 000 cells approximately, such as islets of Langerhans, at least two sorting microchannels arranged in parallel at the outlet of said unit being respectively designed so as to transport as many categories of sorted clusters continuously to a unit for encapsulation of the latter, also formed in said array.
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