A method of manufacturing a polymer film having a microphase-separated morphology includes a film-forming step in which a solution containing a block copolymer having two or more types of polymer chains which are mutually incompatible and are bonded to each other, a first solvent and a second solvent having a higher boiling point by at least 15° C. than the first solvent is applied onto a substrate and the first solvent is removed to form a film; a heating step in which the film is heated at a temperature which is higher than the boiling point of the first solvent but is lower than the boiling point of the second solvent to make the film formed have a microphase-separated morphology; and a solvent removal step in which the second solvent is removed from the film having the microphase-separated morphology to form the polymer film having the microphase-separated morphology.