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US08357836B2 Agrobacterium-mediated method for producing transformed maize or rice 有权
农杆菌介导的转化玉米或水稻的生产方法

  • Patent Title: Agrobacterium-mediated method for producing transformed maize or rice
  • Patent Title (中): 农杆菌介导的转化玉米或水稻的生产方法
  • Application No.: US12935525
    Application Date: 2009-03-24
  • Publication No.: US08357836B2
    Publication Date: 2013-01-22
  • Inventor: Yuji IshidaYukoh Hiei
  • Applicant: Yuji IshidaYukoh Hiei
  • Applicant Address: JP Tokyo
  • Assignee: Japan Tobacco Inc.
  • Current Assignee: Japan Tobacco Inc.
  • Current Assignee Address: JP Tokyo
  • Agency: Birch, Stewart, Kolasch & Birch, LLP
  • Priority: JP2008-094049 20080331
  • International Application: PCT/JP2009/055791 WO 20090324
  • International Announcement: WO2009/122962 WO 20090810
  • Main IPC: A01H1/00
  • IPC: A01H1/00
Agrobacterium-mediated method for producing transformed maize or rice
Abstract:
An Agrobacterium-mediated method for producing transformed maize or rice, culturing an Agrobacterium-inoculated immature embryo with a coculture medium and a regeneration step for culturing the immature embryo with a regeneration medium either without callus growth or after callus growth culture to regenerate whole transformed maize or rice. The method further includes a transformation enhancement and the method does not include any selection step based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration.
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