公开(公告)号：US08357836B2

公开(公告)日：2013-01-22

申请号：US12935525

申请日：2009-03-24

申请人： Yuji Ishida ,  Yukoh Hiei

发明人： Yuji Ishida ,  Yukoh Hiei

IPC分类号： A01H1/00

CPC分类号： C12N15/8205 ,  C12N15/821 ,  C12N15/8212

摘要： An Agrobacterium-mediated method for producing transformed maize or rice, culturing an Agrobacterium-inoculated immature embryo with a coculture medium and a regeneration step for culturing the immature embryo with a regeneration medium either without callus growth or after callus growth culture to regenerate whole transformed maize or rice. The method further includes a transformation enhancement and the method does not include any selection step based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration.

摘要翻译： 用于生产转化的玉米或水稻的农杆菌介导的方法，用共培养培养基培养土壤杆菌接种的未成熟胚胎和用再生培养基培养未成熟胚胎的再生步骤，无需愈伤组织生长或在愈伤组织生长培养后再生整个转化的玉米 或米饭。 该方法还包括转化增强，并且该方法不包括基于在从共培养至再生的任何步骤中被农杆菌引入的核酸的性质的任何选择步骤。

公开(公告)号：US20120124696A1

公开(公告)日：2012-05-17

申请号：US13387370

申请日：2010-07-29

申请人： Yuji Ishida ,  Yukoh Hiei

发明人： Yuji Ishida ,  Yukoh Hiei

IPC分类号： A01H1/06 ,  C12N15/82

CPC分类号： C12N15/8205

摘要： The method of the present invention includes the step of excising one or more portions selected from a radicle, a germ, and an embryonic axis of a plant tissue inoculated with Agrobacterium after cultivation in a coculture medium. The present invention provides a method of gene introduction that can transform a Triticum plant at high efficiency compared to conventionally known Agrobacterium methods, and provides a method of producing a transformed plant.

摘要翻译： 本发明的方法包括在共培养培养基中培养后切除选自土壤杆菌接种的植物组织的胚根，胚芽和胚轴的一个或多个部分的步骤。 本发明提供了一种基因导入方法，其可以与常规已知的农杆菌方法相比高效地转化小麦植物，并提供了转化植物的生产方法。

公开(公告)号：US20110131685A1

公开(公告)日：2011-06-02

申请号：US12086426

申请日：2006-12-13

申请人： Yuji Ishida ,  Yukoh Hiei ,  Jun Ueki ,  Takeshi Yamamoto

发明人： Yuji Ishida ,  Yukoh Hiei ,  Jun Ueki ,  Takeshi Yamamoto

IPC分类号： C12N15/82

CPC分类号： C12N15/8205

摘要： The present invention provides a method for Agrobacterium-mediated gene transfer into a plant material, which comprises inoculating an Agrobacterium into the plant material in the presence of a powder. In the method of the present invention, the powder at least does not affect living tissues and has one or more properties selected from the group consisting of: being insoluble in water; having an affinity for living tissues; having adsorption properties; and having a surface polarity. The present invention also provides a method for producing a transformed plant, which comprises using the gene transfer method of the present invention.

摘要翻译： 本发明提供了将土壤杆菌介导的基因转移到植物材料中的方法，其包括在粉末存在下将土壤杆菌接种到植物材料中。 在本发明的方法中，所述粉末至少不影响活体组织，并且具有选自下组的一种或多种性质：不溶于水; 对活组织有亲和力; 具有吸附性能; 并具有表面极性。 本发明还提供一种生产转化植物的方法，其包括使用本发明的基因转移方法。

公开(公告)号：US20100132068A1

公开(公告)日：2010-05-27

申请号：US12306163

申请日：2007-06-25

申请人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

发明人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

IPC分类号： A01H1/00 ,  C12N15/63

CPC分类号： C12N15/82 ,  C12N15/8205

摘要： The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.

摘要翻译： 本发明旨在提供用于植物转化的新型载体。 本发明的载体是全长为15kb或更小的粘粒载体，其特征在于：1）它们含有IncP质粒的复制起点，但不含有其他质粒组的任何复制起点; 2）它们含有IncP质粒的trfA1基因; 3）它们含有一个IncP质粒的oriT; 4）它们含有IncP质粒的incC1基因; 5）它们含有λ噬菌体的cos位点，cos位点位于T-DNA外部; 6）它们含有在大肠杆菌中表达的耐药基因和农杆菌属的细菌; 7）它们含有农杆菌属细菌的T-DNA右侧序列; 8）它们含有农杆菌属细菌的T-DNA左边界序列; 9）它们含有位于7）和8之间的植物转化的选择性标记基因并在植物中表达; 和10）它们含有位于7）和8之间的限制性内切核酸酶识别位点，用于克隆外来基因。

公开(公告)号：US07525014B2

公开(公告)日：2009-04-28

申请号：US10363832

申请日：2001-09-07

申请人： Yoshimitsu Takakura ,  Yasuhiro Inoue ,  Shigeru Kuwata ,  Fumiki Tsutsumi ,  Yuji Ishida

发明人： Yoshimitsu Takakura ,  Yasuhiro Inoue ,  Shigeru Kuwata ,  Fumiki Tsutsumi ,  Yuji Ishida

IPC分类号： C12N15/09 ,  C12N15/31 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8282 ,  C07K14/21

摘要： It is the object of the present invention to provide disease-resistant plants which have been transformed to cause an effective defense reaction, and methods for producing the same.The present invention provides expression cassettes comprising a promoter capable of promoting a constitutive, inducible, or organ- or phase-specific gene expression, and a gene, under the control of said promoter, encoding an elicitor protein.

摘要翻译： 本发明的目的是提供已经转化以产生有效防御反应的抗病植物及其制备方法。 本发明提供了包含能够促进组成型，诱导型或器官或相位特异性基因表达的启动子的表达盒，以及在所述启动子控制下编码引发蛋白的基因。

公开(公告)号：US20080219372A1

公开(公告)日：2008-09-11

申请号：US12027035

申请日：2008-02-06

申请人： Kazuyuki Hori ,  Yuji Ishida ,  Shouhei Murakami ,  Kenji Yanagi

发明人： Kazuyuki Hori ,  Yuji Ishida ,  Shouhei Murakami ,  Kenji Yanagi

IPC分类号： H04L5/12

CPC分类号： H04L27/2614 ,  H04L27/2624

摘要： An OFDM modulator having a peak factor reduction function. The OFDM modulator has a peak factor reduction unit between an IFFT unit and a guard interval insertion unit thereof. The peak factor reduction unit converts a complex signal X1 outputted from the IFFT unit into a complex signal X2 with a reduced peak factor based on subcarrier map information. The peak factor reduction unit generates a peak factor reduction signal by a linear combination of complex exponential functions that correspond to subcarrier frequencies to be used for wave transmission, as bases. The peak factor reduction signal is derived by repetition of, for example, a weighted least squares method or convolution processing by a fast Fourier transform.

摘要翻译： 一种具有峰值因子减小函数的OFDM调制器。 OFDM调制器在IFFT单元与其保护间隔插入单元之间具有峰值因子减小单元。 峰值因子降低单元基于副载波映射信息将从IFFT单元输出的复信号X 1以具有降低的峰值因子的复信号X 2转换。 峰值因子降低单元通过对应于用于波传输的副载波频率的复指数函数的线性组合作为基础产生峰值因子减小信号。 通过重复例如加权最小二乘法或快速傅里叶变换的卷积处理来导出峰值因子降低信号。

公开(公告)号：US07396977B2

公开(公告)日：2008-07-08

申请号：US10493537

申请日：2002-10-23

申请人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

发明人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

IPC分类号： C12N15/82

CPC分类号： C12N9/1294 ,  C12N15/8269

摘要： The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

摘要翻译： 本发明涉及C4植物的转化。 它也涉及C4植物中外源基因的高水平表达。 更具体地，本发明涉及通过实现构成C4光合途径的酶的高水平表达来产生在低温下保持优异光合能力的C4植物。 在本发明中，使用包含启动子，C4植物基因组基因的表达盒，在所述启动子的控制下，编码构成光合途径的酶和终止子的转化C4植物。 编码构成光合途径的酶的C4植物基因组基因优选为C4植物基因组衍生的PPDK基因或其修饰形式。 本发明特别可用于改进具有PPDK的C4植物的生产（特别是在改善低温条件下玉米的生产中）。

公开(公告)号：US6091305A

公开(公告)日：2000-07-18

申请号：US369200

申请日：1999-08-06

申请人： Yoshinori Nagoya ,  Yuji Ishida ,  Ken Takei

发明人： Yoshinori Nagoya ,  Yuji Ishida ,  Ken Takei

IPC分类号： H03L7/089 ,  H03L7/093 ,  H03L7/18 ,  H03L7/00

CPC分类号： H03L7/18 ,  H03L7/0891 ,  H03L7/093

摘要： It is an object of the present invention to reduce the amount of spurious noise generated by an emission line spectrum of a signal propagating around the PLL of a PLL frequency synthesizer carrying out digital-signal processing.In order to achieve the object described above, the present invention provides a PLL frequency synthesizer with the following configuration.In a PLL frequency synthesizer having a charge-pump circuit, a waveform converter is provided at a stage behind the charge-pump circuit. The waveform converter converts the voltage waveform on a time axis of a rectangular wave output by the charge-pump circuit into a waveform which: is symmetrical with respect to a predetermined point of time; oscillates so as to have no direct-current component; and has a maximum value of the absolute values of maximums of wave heights thereof located at the center wherein the absolute value decreases uniformly as the wave height is separated farther away from the center. As a result, the amplitudes of a limited number of emission line spectra forming a spurious signal superposed on a signal propagating around the PLL can be made uniform and a maximum thereof can be held at a low value, allowing the direct-current component to be eliminated from the signal propagating around the PLL.

摘要翻译： 本发明的目的是减少由执行数字信号处理的PLL频率合成器的PLL周围传播的信号的发射线谱产生的杂散噪声量。 为了实现上述目的，本发明提供一种具有以下配置的PLL频率合成器。 在具有电荷泵电路的PLL频率合成器中，在电荷泵电路后面的一级提供波形转换器。 波形转换器将由电荷泵电路输出的矩形波的时间轴上的电压波形转换为相对于预定时间点对称的波形; 振荡，不具有直流分量; 并且具有位于中心处的波高的绝对值的绝对值的最大值，其中绝对值随波高离中心距离而均匀地减小。 结果，可以使形成叠加在围绕PLL周围的信号叠加的寄生信号的有限数量的发射线谱的幅度是均匀的，并且其最大值可以保持在低值，允许直流分量为 从PLL周围传播的信号中消除。

公开(公告)号：US5963099A

公开(公告)日：1999-10-05

申请号：US810435

申请日：1997-03-04

申请人： Yoshinori Nagoya ,  Yuji Ishida ,  Ken Takei

发明人： Yoshinori Nagoya ,  Yuji Ishida ,  Ken Takei

IPC分类号： H03L7/089 ,  H03L7/093 ,  H03L7/18 ,  H03B19/00 ,  H03K5/00

CPC分类号： H03L7/18 ,  H03L7/0891 ,  H03L7/093

摘要： It is an object of the present invention to reduce the amount of spurious noise generated by an emission line spectrum of a signal propagating around the PLL of a PLL frequency synthesizer carrying out digital-signal processing.In order to achieve the object described above, the present invention provides a PLL frequency synthesizer with the following configuration.In a PLL frequency synthesizer having a charge-pump circuit, a waveform converter is provided at a stage behind the charge-pump circuit. The waveform converter converts the voltage waveform on a time axis of a rectangular wave output by the charge-pump circuit into a waveform which: is symmetrical with respect to a predetermined point of time; oscillates so as to have no direct-current component; and has a maximum value of the absolute values of maximums of wave heights thereof located at the center wherein the absolute value decreases uniformly as the wave height is separated farther away from the center. As a result, the amplitudes of a limited number of emission line spectra forming a spurious signal superposed on a signal propagating around the PLL can be made uniform and a maximum thereof can be held at a low value, allowing the direct-current component to be eliminated from the signal propagating around the PLL.

公开(公告)号：US5757244A

公开(公告)日：1998-05-26

申请号：US606072

申请日：1996-02-23

申请人： Youji Nonaka ,  Yuji Ishida ,  Youji Suwa ,  Toshikazu Hiramoto

发明人： Youji Nonaka ,  Yuji Ishida ,  Youji Suwa ,  Toshikazu Hiramoto

IPC分类号： H03D7/16 ,  H03L1/02

CPC分类号： H03L1/025 ,  H03D7/165 ,  H03L1/028

摘要： In a portable telephone which uses a CPU, a memory, a temperature sensor, a D/A converter, and an A/d converter as control elements, and which has an oscillation circuit including a crystal resonator and a variable-capacitance diode, temperature compensation of the output frequency is made by using these existing control elements. The memory is previously stored with control information for correcting an output frequency drift of the portable telephone caused by a temperature change. The temperature of the oscillation circuit is detected with the temperature sensor and converted into a digital value in the A/D converter. The CPU reads control information corresponding to the detected temperature from the memory and applies it to the variable-capacitance diode of the oscillation circuit through the D/A converter, thereby maintaining the output frequency at a constant level. Also disclosed is a method of efficiently calculating an oscillation frequency of a crystal resonator with a reduced number of points of measurement for a temperature characteristic of the crystal resonator. A method of accurately carrying out the above-described temperature compensation is also disclosed.

摘要翻译： 在使用CPU，存储器，温度传感器，D / A转换器和A / D转换器作为控制元件的便携式电话中，具有包括晶体谐振器和可变电容二极管的振荡电路，温度 通过使用这些现有的控制元件来补偿输出频率。 存储器预先存储有用于校正由温度变化引起的便携式电话的输出频率漂移的控制信息。 用温度传感器检测振荡电路的温度，并在A / D转换器中转换为数字值。 CPU从存储器读取对应于检测到的温度的控制信息，并通过D / A转换器将其施加到振荡电路的可变电容二极管，从而将输出频率保持在恒定水平。 还公开了一种利用晶体谐振器的温度特性减少的测量点数来有效地计算晶体谐振器的振荡频率的方法。 还公开了一种精确地执行上述温度补偿的方法。