A multi-channel arrayed isosbestic wavelength detection system comprises an arrayed light source board, an arrayed photoelectric sensor board, and an intermediate system frame. The arrayed light source board and arrayed photoelectric sensor board are assembled at opposite sides of the intermediate system frame. In addition, the arrayed light source system has a plurality of light-emitting elements, each of which comprises two monochromatic light sources that provide main wavelength and reference wavelength respectively, and the two wavelengths are isosbestic wavelengths. The arrayed photoelectric sensor system has a plurality of photoelectric sensors, which are aligned at fixed positions in one-to-one correspondence with the light-emitting elements.