Invention Grant
- Patent Title: Fusion proteins
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Application No.: US12862948Application Date: 2010-08-25
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Publication No.: US08399400B2Publication Date: 2013-03-19
- Inventor: Keith Foster , John Chaddock , Philip Marks , Patrick Stancombe , Kei Roger Aoki , Joseph Francis , Lance Steward
- Applicant: Keith Foster , John Chaddock , Philip Marks , Patrick Stancombe , Kei Roger Aoki , Joseph Francis , Lance Steward
- Applicant Address: GB Abingdon US CA Irvine
- Assignee: Syntaxin, Ltd.,Allergan, Inc.
- Current Assignee: Syntaxin, Ltd.,Allergan, Inc.
- Current Assignee Address: GB Abingdon US CA Irvine
- Agency: Roylance, Abrams, Berdo & Goodman, L.L.P.
- Priority: GB0426394.3 20041201; GB0504964.8 20050310
- Main IPC: C07K14/00
- IPC: C07K14/00 ; C07H21/02

Abstract:
A single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a nociceptive sensory afferent; a dynorphin Targeting Moiety that is capable of binding to a Binding Site on the nociceptive sensory afferent, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the nociceptive sensory afferent; a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the dynorphin Targeting Moiety; and a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent. Nucleic acid sequences encoding the polypeptide fusion proteins, methods of preparing same and uses thereof are also described.
Public/Granted literature
- US20110027256A1 FUSION PROTEINS Public/Granted day:2011-02-03
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