发明授权
- 专利标题: Modified glucose dehydrogenase gene
- 专利标题(中): 改良葡萄糖脱氢酶基因
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申请号: US12810213申请日: 2008-12-26
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公开(公告)号: US08445221B2公开(公告)日: 2013-05-21
- 发明人: Michinari Honda , Ryo Takenaka , Fuminao Kobayashi
- 申请人: Michinari Honda , Ryo Takenaka , Fuminao Kobayashi
- 申请人地址: JP Hiroshima
- 专利权人: Ikeda Food Research Co., Ltd.
- 当前专利权人: Ikeda Food Research Co., Ltd.
- 当前专利权人地址: JP Hiroshima
- 代理机构: Wenderoth, Lind & Ponack, L.L.P.
- 优先权: JP2007-340477 20071228
- 国际申请: PCT/JP2008/073689 WO 20081226
- 国际公布: WO2009/084616 WO 20090709
- 主分类号: C12Q1/54
- IPC分类号: C12Q1/54 ; C12N9/00 ; C12N9/02 ; C12N1/20 ; C07H21/04
摘要:
[Object] To provide an FAD-conjugated glucose dehydrogenase having a higher specificity for glucose.[Means for Resolution] A modified glucose dehydrogenase (GLD) which includes a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 37, 69, 72, 73, 76, 78, 102, 217, 228, 240, 356, 407, 424, 437, 527, and 530 in an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1, and has a decreased ratio of activity for xylose/activity for glucose as compared with the wild-type GLD; a polynucleotide encoding the modified GLD; a recombinant vector containing the polynucleotide; a transformed cell produced by using the recombinant vector; etc.
公开/授权文献
- US20100323378A1 MODIFIED GLUCOSE DEHYDROGENASE GENE 公开/授权日:2010-12-23