发明授权
- 专利标题： Method of detecting methylated cytosine
- 专利标题（中）： 检测甲基化胞嘧啶的方法
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申请号： US12674648申请日： 2008-08-20
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公开(公告)号： US08465919B2公开(公告)日： 2013-06-18
- 发明人: Masahiro Kajita , Noriko Oka , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
- 申请人： Masahiro Kajita , Noriko Oka , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
- 申请人地址： JP Kobe
- 专利权人： Sysmex Corporation
- 当前专利权人： Sysmex Corporation
- 当前专利权人地址： JP Kobe
- 代理机构： Sughrue Mion, PLLC
- 优先权： JP2007-217263 20070823
- 国际申请： PCT/JP2008/064826 WO 20080820
- 国际公布： WO2009/025296 WO 20090226
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/00 ; C07H21/02 ; C07H21/04
摘要：
The method for detecting a methylated cytosine of the present invention comprises the steps of: hybridizing a sample DNA with an oligonucleotide which can hybridize with a region of the sample DNA containing a cytosine suspected of being methylated and has an a basic site at the position complementary to the cytosine; reacting the hybridized sample DNA obtained in the previous step with an oxidizing agent to oxidize the cytosine when it is methylated; and detecting the oxidized methylated cytosine.
公开/授权文献
- US20100240042A1 METHOD OF DETECTING METHYLATED CYTOSINE 公开/授权日：2010-09-23
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