发明授权
US08470592B2 Isolation and characterization of Schizochytrium aggregatum cellobiohydrolase I (Cbh 1)
有权
裂殖壶菌纤维二糖水解酶I（Cbh 1）的分离和表征
- 专利标题： Isolation and characterization of Schizochytrium aggregatum cellobiohydrolase I (Cbh 1)
- 专利标题（中）： 裂殖壶菌纤维二糖水解酶I（Cbh 1）的分离和表征
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申请号： US13003199申请日： 2009-07-07
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公开(公告)号： US08470592B2公开(公告)日： 2013-06-25
- 发明人: Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
- 申请人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
- 申请人地址： US NH Lebanon
- 专利权人： Mascoma Corporation
- 当前专利权人： Mascoma Corporation
- 当前专利权人地址： US NH Lebanon
- 代理机构： Sterne, Kessler, Goldstein & Fox P.L.L.C.
- 国际申请： PCT/US2009/003972 WO 20090707
- 国际公布： WO2010/005553 WO 20100114
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N9/00 ; C07H21/04 ; C07K14/00
摘要：
The present invention provides for the isolation and characterization of the cbh1 gene from Schizochytrium aggregatum. In particular, the present invention provides for the nucleic acid and amino acid sequences of Schizochytrium aggregatum cbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression of Schizochytrium aggregatum Cbh1 in host cells, including yeast, e.g., Saccharomyces cerevisiae. Expression of Schizochytrium aggregatum Cbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression in Saccharomyces cerevisiae is in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novel Schizochytrium aggregatum Cbh1 gene can utilized in a consolidated bioprocessing system.