Invention Grant
US08501925B2 Nucleic acid modules for expression and tagging of membrane proteins and methods of use
失效
用于膜蛋白表达和标记的核酸模块及其使用方法
- Patent Title: Nucleic acid modules for expression and tagging of membrane proteins and methods of use
- Patent Title (中): 用于膜蛋白表达和标记的核酸模块及其使用方法
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Application No.: US12652395Application Date: 2010-01-05
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Publication No.: US08501925B2Publication Date: 2013-08-06
- Inventor: Li Lin , John Pang , Wen Wei , Edward G. Lakatta
- Applicant: Li Lin , John Pang , Wen Wei , Edward G. Lakatta
- Applicant Address: US DC Washington
- Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Resources
- Current Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Resources
- Current Assignee Address: US DC Washington
- Agency: Klarquist Sparkman, LLP
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12Q1/68
Abstract:
Described herein are nucleic acid modules for cloning, expression and tagging of eukaryotic membrane proteins. The nucleic acid modules include a receptor for advanced glycation end products (RAGE) signal sequence, a nucleic acid sequence encoding a tag and a multiple cloning sequence (MCS). Any membrane protein of interest can be cloned into the MCS for expression in cells. The nucleic acid modules can encode any type of tag, such as an epitope tag or affinity tag. The nucleic acid modules disclosed herein can be used to express any type of membrane protein and are particularly suited to the expression and tagging of Type I and Type III membrane proteins.
Public/Granted literature
- US20100173307A1 NUCLEIC ACID MODULES FOR EXPRESSION AND TAGGING OF MEMBRANE PROTEINS AND METHODS OF USE Public/Granted day:2010-07-08
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