发明授权
- 专利标题: Method of inducing differentiation of embryonic stem cells into hemangioblast
- 专利标题(中): 诱导胚胎干细胞分化为成血管细胞的方法
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申请号: US12858747申请日: 2010-08-18
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公开(公告)号: US08507275B2公开(公告)日: 2013-08-13
- 发明人: Yong-Mahn Han , Sang-Wook Park , Eun-Young Lee
- 申请人: Yong-Mahn Han , Sang-Wook Park , Eun-Young Lee
- 申请人地址: KR Daejeon
- 专利权人: Korea Advanced Institute of Science and Technology (Kaist)
- 当前专利权人: Korea Advanced Institute of Science and Technology (Kaist)
- 当前专利权人地址: KR Daejeon
- 代理机构: Knobbe, Martens, Olson & Bear, LLP
- 主分类号: C12N5/00
- IPC分类号: C12N5/00 ; C12N5/02 ; C12N5/08 ; A01N61/00 ; A01N37/18
摘要:
The present invention relates to a composition for inducing embryonic stem cell differentiation comprising a MEK/ERK (mitogen-activated protein kinase kinase/extracellular regulated kinase) signal transduction inhibitor and BMP (bone morphogenetic protein), and a method for inducing differentiation of embryonic stem cells into mesodermal cells using the same. Further, the mesodermal cells obtained by the above method are able to differentiate into various mesenchymal tissue cells. In particular, the present invention relates to a method for inducing differentiation into hemangioblast by culturing the mesodermal cells obtained by the above method in the presence of VEGF (vascular endothelial cell growth factor) and bFGF (basic fibroblast growth factor). The differentiated hemangioblasts can be further differentiated into vascular endothelial cells, vascular smooth muscle cells, and hematopoietic stem cells under various culture conditions.