发明授权
US08513399B2 Primers for PCR amplification comprising a basic parts within the primer sequences
有权
用于PCR扩增的引物，其包含引物序列内的基本部分
- 专利标题： Primers for PCR amplification comprising a basic parts within the primer sequences
- 专利标题（中）： 用于PCR扩增的引物，其包含引物序列内的基本部分
-
申请号： US12681754申请日： 2008-10-02
-
公开(公告)号： US08513399B2公开(公告)日： 2013-08-20
- 发明人: Hyun Bae Kim , Seong Youl Kim , Jun Mo Gil , Hae Joon Park , Han Oh Park
- 申请人： Hyun Bae Kim , Seong Youl Kim , Jun Mo Gil , Hae Joon Park , Han Oh Park
- 申请人地址： KR Daejeon
- 专利权人： Bioneer Corporation
- 当前专利权人： Bioneer Corporation
- 当前专利权人地址： KR Daejeon
- 代理机构： Rothwell, Figg, Ernst & Manbeck, P.C.
- 优先权： KR10-2007-0100507 20071005
- 国际申请： PCT/KR2008/005821 WO 20081002
- 国际公布： WO2009/045067 WO 20090409
- 主分类号： C07H21/04
- IPC分类号： C07H21/04 ; C07H19/04 ; C12Q1/68 ; C12P19/34
摘要：
The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.
公开/授权文献
信息查询
